Ex vivo susceptibilities of Plasmodium vivax isolates from the China-Myanmar border to antimalarial drugs and association with polymorphisms in Pvmdr1 and Pvcrt-o genes

PLoS Neglected Tropical Diseases
Jiangyan LiLiwang Cui

Abstract

Vivax malaria is an important public health problem in the Greater Mekong Subregion (GMS), including the China-Myanmar border. Previous studies have found that Plasmodium vivax has decreased sensitivity to antimalarial drugs in some areas of the GMS, but the sensitivity of P. vivax to antimalarial drugs is unclear in the China-Myanmar border. Here, we investigate the drug sensitivity profile and genetic variations for two drug resistance related genes in P. vivax isolates to provide baseline information for future drug studies in the China-Myanmar border. A total of 64 P. vivax clinical isolates collected from the China-Myanmar border area were assessed for ex vivo susceptibility to eight antimalarial drugs by the schizont maturation assay. The medians of IC50 (half-maximum inhibitory concentrations) for chloroquine, mefloquine, pyronaridine, piperaquine, quinine, artesunate, artemether, dihydroartemisinin were 84.2 nM, 34.9 nM, 4.0 nM, 22.3 nM, 41.4 nM, 2.8 nM, 2.1 nM and 2.0 nM, respectively. Twelve P. vivax clinical isolates were found over the cut-off IC50 value (220 nM) for chloroquine resistance. In addition, sequence polymorphisms in pvmdr1 (P. vivax multidrug resistance-1), pvcrt-o (P. vivax chloroquine resistance trans...Continue Reading

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Citations

Dec 29, 2020·International Journal for Parasitology. Drugs and Drug Resistance·Marcelo U FerreiraJosé Pedro Gil
May 7, 2021·International Journal for Parasitology. Drugs and Drug Resistance·Lucas E BuyonManoj T Duraisingh
Nov 19, 2021·Frontiers in Cellular and Infection Microbiology·Weilin ZengZhaoqing Yang

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Datasets Mentioned

BETA
AF314649

Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad
CLUSTAL X
DNASTAR
SPSS
Graphpad Prism
Primer Premier

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