Ex vivo Whole-cell Recordings in Adult Drosophila Brain

Bio-protocol
Alexa J RoemmichDiane K O'Dowd

Abstract

Cost-effective and efficient, the fruit fly (Drosophila melanogaster) has been used to make many key discoveries in the field of neuroscience and to model a number of neurological disorders. Great strides in understanding have been made using sophisticated molecular genetic tools and behavioral assays. Functional analysis of neural activity was initially limited to the neuromuscular junction (NMJ) and in the central nervous system (CNS) of embryos and larvae. Elucidating the cellular mechanisms underlying neurological processes and disorders in the mature nervous system have been more challenging due to difficulty in recording from neurons in adult brains. To this aim we developed an ex vivo preparation in which a whole brain is isolated from the head capsule of an adult fly and placed in a recording chamber. With this preparation, whole cell recording of identified neurons in the adult brain can be combined with genetic, pharmacological and environmental manipulations to explore cellular mechanisms of neuronal function and dysfunction. It also serves as an important platform for evaluating the mechanism of action of new therapies identified through behavioral assays for treating neurological diseases. Here we present our proto...Continue Reading

References

Dec 20, 2003·Science·Rachel I WilsonGilles Laurent
Jan 10, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Huaiyu Gu, Diane K O'Dowd
Sep 6, 2008·Current Biology : CB·Vasu SheebaTodd C Holmes
Sep 9, 2008·Nature Neuroscience·François V BolducTim Tully
Nov 11, 2008·Journal of Visualized Experiments : JoVE·Huaiyu Gu, Diane K O'Dowd
Oct 12, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lei SunRobert Reenan
May 9, 2014·Journal of Neurophysiology·Ryan J SchutteDiane K O'Dowd
Feb 5, 2016·Journal of Neurophysiology·Soleil S SchutteDiane K O'Dowd
Apr 15, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Katherine I Nagel, Rachel I Wilson
Jun 7, 2016·Neuron·Isaac Cervantes-SandovalRonald L Davis

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