PMID: 11332032May 3, 2001Paper

Exacerbating effect of vitamin E supplementation on DNA damage induced in cultured human normal fibroblasts by UVA radiation

Photochemistry and Photobiology
S NocentiniS Surgis

Abstract

The effects of vitamin E supplementation were evaluated in cultured human normal fibroblasts exposed to ultraviolet A radiation (320-380 nm) (UVA). Cells were incubated in medium containing alpha-tocopherol, alpha-tocopherol acetate or the synthetic analog Trolox for 24 h prior to UVA exposure. DNA damage in the form of frank breaks and alkali-labile sites, collectively termed single-strand breaks (SSB), was assayed by the technique of single cell gel electrophoresis (comet assay), immediately following irradiation or after different repair periods. The generation of hydrogen peroxide (H2O2) and superoxide ion (O2.-) was measured by flow cytometry through the oxidation of indicators into fluorescent dyes. It was observed that pretreatment of cells with any form of vitamin E resulted in an increased susceptibility to the photoinduction of DNA SSB and in a longer persistence of damage, whereas no significant change was observed in the production of H2O2 and O2.- reactive oxygen species, compared to untreated controls. These findings indicate that in human normal fibroblasts, exogenously added vitamin E exerts a promoting activity on DNA damage upon UVA irradiation and might lead to increased cytotoxic and mutagenic risks.

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Citations

Nov 16, 2004·Journal of Photochemistry and Photobiology. B, Biology·Marianna BudaiPál Gróf
Sep 24, 2005·Journal of Medicinal Food·Richa Gupta, S J S Flora
Mar 4, 2005·Biological & Pharmaceutical Bulletin·Yanji XuSantian Cui
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Aug 30, 2011·Journal of Hazardous Materials·Ahmed E Abdel MoneimSaleh Al-Quraishy
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Mar 27, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bernd HantkeHarald Tschesche

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