Examination of a modified cell cycle synchronization method and bovine nuclear transfer using synchronized early G1 phase fibroblast cells

Theriogenology
Manami UrakawaYoshito Aoyagi

Abstract

Somatic cell nuclear transfer has a low success rate, due to a high incidence of fetal loss and increased perinatal morbidity/mortality. One factor that may affect the successful development of nuclear transfer embryos is the cell cycle stage of the donor cell. In order to establish a cell cycle synchronization method that can consistently produce cloned embryos and offspring, we examined the effects of different combinations of three cell treatments on the recovery rate of mitotic phase cells using bovine fetal fibroblasts. In the first experiment, we examined the recovery rate of mitotic phase cells by a combination of treatment with a metaphase arrestant (1 microM 2-methoxyestradiol), shaking the plate and selecting cells with a diameter of 20 microns. As a result, 99% of mitotic phase cells were recovered by repeating the combined treatment of metaphase arrestant and shaking, and collection of cells with a specific diameter. In the second experiment, nuclear transfer was carried out using early G1 phase cells by choosing pairs of bridged cells derived from mitotic phase cells recovered by the combined treatment of 1 microM 2-methoxyestradiol and shaking, and collection of cells with a diameter of 20 microns. The reconstruct...Continue Reading

References

Dec 16, 1994·Science·L H Hartwell, M B Kastan
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·R J D'AmatoE Hamel
Nov 12, 1996·Biochemical and Biophysical Research Communications·H AttallaL C Andersson
Feb 27, 1997·Nature·I WilmutK H Campbell
Dec 16, 1998·Science·Y KatoY Tsunoda
May 20, 1999·Nature Biotechnology·A BaguisiY Echelard
Sep 25, 1999·Molecular Reproduction and Development·V ZakhartchenkoE Wolf
Aug 19, 2000·Science·A OnishiA C Perry
Sep 19, 2000·Nature·I A PolejaevaK H Campbell
Dec 4, 2001·Nature Biotechnology·P KasinathanJ M Robl
Dec 26, 2001·Biology of Reproduction·Y HeymanJ P Renard
Feb 23, 2002·Nature·T ShinM Westhusin
Mar 22, 2002·Biology of Reproduction·John GibbonsSteve Stice
Mar 30, 2002·Nature Biotechnology·Patrick ChesnéJean-Paul Renard
Jun 13, 2003·Biology of Reproduction·Kimiko InoueAtsuo Ogura
Aug 9, 2003·Nature·Cesare GalliGiovanna Lazzari
Apr 1, 1963·Experimental Cell Research·T TERASIMA, L J TOLMACH

❮ Previous
Next ❯

Citations

Sep 13, 2007·Stem Cell Reviews·Andrew J FrenchAlan O Trounson
May 21, 2011·The Journal of Reproduction and Development·Satoshi AkagiSeiya Takahashi
Sep 10, 2008·Yi chuan = Hereditas·Wei-Hua DuDong Wang
Sep 12, 2013·Cellular Reprogramming·D N Wells
Dec 25, 2007·Cloning and Stem Cells·Atsushi IdetaKazuhiro Saeki
Mar 14, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Satoshi AkagiTakashi Nagai
Jul 7, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Shinya Watanabe, Takashi Nagai
Jul 20, 2016·PloS One·Ji Hye LeeMin Kyu Kim
Nov 17, 2010·Izvestiia Akademii nauk. Seriia biologicheskaia·G P MalenkoO I Stepanov
Nov 21, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Atsuo OguraTeruhiko Wakayama

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.