Examination of cytotoxicity and mutagenicity of AH26 and AH Plus sealers

International Endodontic Journal
Ivana MiletićM Osmak

Abstract

To study in vitro the cytotoxic and mutagenic effects of AH26 and AH Plus. Cytotoxic effects on Chinese hamster V79 cells were determined by counting viable cells following incubation with eluations of AH26 and AH Plus. In one set of experiments, the materials were mixed, set for 1 h and then eluted with dimethyl sulphoxide (DMSO) for 1 h, 24 h and 7 days. In the other set, AH26 and AH Plus were mixed and set for 1 h, 24 h and 7 days in physiological saline then crushed and eluted in DMSO for 24 h. The cytotoxic effects of these eluates were evaluated. Three concentrations were chosen to examine the mutagenic effects of AH26 and AH Plus: 5.57, 16.7 and 55.7 microg mL(-1). The structural chromosomal aberration analysis and micronucleus test were performed on human lymphocytes according to standard procedures. Dose-response curves of cell survival were obtained. Both materials were shown to be cytotoxic in doses larger than 55.7 microg mL(-1), except for AH26, after 7 days setting time. AH Plus was also shown to be toxic in concentrations of 16.7 microg mL(-1), except after 7 days setting time. Neither AH26 nor AH Plus induced a significant increase of chromosomal aberrations or micronuclei induction at any setting time or concen...Continue Reading

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Citations

Feb 2, 2012·Biological & Pharmaceutical Bulletin·Yoichi KawasakiToshiaki Sendo
Mar 12, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Amer Z AlaneziQiang Zhu
Jan 25, 2011·International Endodontic Journal·D Silva-HerzogP Nelson-Filho
Feb 23, 2010·Journal of Endodontics·Débora Silveira BrasilFrank Ferreira Silveira
Sep 29, 2012·Brazilian Oral Research·Miriam Zaccaro ScelzaGutemberg Gomes Alves
Jul 4, 2019·Dentistry Journal·Alexander BrezhnevJukka P Matinlinna
Jun 8, 2021·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Hilal ErdoganFunda Kont Cobankara

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