Examination of processed vegetable foods for the presence of common DNA sequences of genetically modified tomatoes

Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
Mamiko KitagawaHiroshi Akiyama

Abstract

The contamination of processed vegetable foods with genetically modified tomatoes was investigated by the use of qualitative PCR methods to detect the cauliflower mosaic virus 35S promoter (P35S) and the kanamycin resistance gene (NPTII). DNA fragments of P35S and NPTII were detected in vegetable juice samples, possibly due to contamination with the genomes of cauliflower mosaic virus infecting juice ingredients of Brassica species and soil bacteria, respectively. Therefore, to detect the transformation construct sequences of GM tomatoes, primer pairs were designed for qualitative PCR to specifically detect the border region between P35S and NPTII, and the border region between nopaline synthase gene promoter and NPTII. No amplification of the targeted sequences was observed using genomic DNA purified from the juice ingredients. The developed qualitative PCR method is considered to be a reliable tool to check contamination of products with GM tomatoes.

References

May 28, 2003·Toxicology·Zhang-Liang ChenLi-Jia Qu
Jul 5, 2007·Journal of Agricultural and Food Chemistry·Hiroshi AkiyamaTamio Maitani
Jun 11, 2009·Journal of Integrative Plant Biology·Lin ChengGang Lu
Oct 4, 2011·Biological & Pharmaceutical Bulletin·Kosuke NakamuraReiko Teshima
Oct 17, 2012·Journal of the Science of Food and Agriculture·Chao YangLitao Yang
Nov 8, 2012·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Hiroshi AkiyamaReiko Teshima
Nov 1, 2013·Biological & Pharmaceutical Bulletin·Kosuke NakamuraTomoko Nishimaki-Mogami

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Citations

Apr 28, 2020·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Hiromasa YamauchiMasami Harada

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