Examination of taxonomic uncertainties surrounding Brucella abortus bv. 7 by phenotypic and molecular approaches

Applied and Environmental Microbiology
Bruno Garin-BastujiAdrian M Whatmore

Abstract

Brucella taxonomy is perpetually being reshuffled, at both the species and intraspecies levels. Biovar 7 of Brucella abortus was suspended from the Approved Lists of Bacterial Names Brucella classification in 1988, because of unpublished evidence that the reference strain 63/75 was a mixture of B. abortus biovars 3 and 5. To formally clarify the situation, all isolates previously identified as B. abortus bv. 7 in the AHVLA and ANSES strain collections were characterized by classical microbiological and multiple molecular approaches. Among the 14 investigated strains, including strain 63/75, only four strains, isolated in Kenya, Turkey, and Mongolia, were pure and showed a phenotypic profile in agreement with the former biovar 7, particularly agglutination with both anti-A/anti-M monospecific sera. These results were strengthened by molecular strategies. Indeed, genus- and species-specific methods allowed confirmation that the four pure strains belonged to the B. abortus species. The combination of most approaches excluded their affiliation with the recognized biovars (biovars 1 to 6 and 9), while some suggested that they were close to biovar 3.These assays were complemented by phylogenetic and/or epidemiological methods, such a...Continue Reading

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Citations

Apr 14, 2016·Infectious Diseases of Poverty·Jun-Ying MaGong-Hua Lin
Sep 23, 2014·International Journal of Systematic and Evolutionary Microbiology·Adrian M WhatmoreNatalia E Schlabritz-Loutsevitch
Mar 21, 2015·BioMed Research International·Denis Rwabiita MugiziUlf Magnusson
Oct 20, 2018·PLoS Neglected Tropical Diseases·Jennifer MesureurAnne Keriel
Jun 15, 2019·Microbiology Resource Announcements·M Sadequl IslamM Ariful Islam
Mar 11, 2017·Journal of the South African Veterinary Association·Songelwayo L ChisiHenriette Van Heerden
Feb 6, 2017·BMC Microbiology·Giuliano GarofoloJeffrey T Foster
Jan 11, 2020·Frontiers in Microbiology·Juan M García LoboFélix J Sangari
Apr 20, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Adrian M Whatmore, Jeffrey T Foster

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