Examination of the genetic basis for sexual dimorphism in the Aedes aegypti (dengue vector mosquito) pupal brain

Biology of Sex Differences
Michael TomchaneyMolly Duman-Scheel

Abstract

Most animal species exhibit sexually dimorphic behaviors, many of which are linked to reproduction. A number of these behaviors, including blood feeding in female mosquitoes, contribute to the global spread of vector-borne illnesses. However, knowledge concerning the genetic basis of sexually dimorphic traits is limited in any organism, including mosquitoes, especially with respect to differences in the developing nervous system. Custom microarrays were used to examine global differences in female vs. male gene expression in the developing pupal head of the dengue vector mosquito, Aedes aegypti. The spatial expression patterns of a subset of differentially expressed transcripts were examined in the developing female vs. male pupal brain through in situ hybridization experiments. Small interfering RNA (siRNA)-mediated knockdown studies were used to assess the putative role of Doublesex, a terminal component of the sex determination pathway, in the regulation of sex-specific gene expression observed in the developing pupal brain. Transcripts (2,527), many of which were linked to proteolysis, the proteasome, metabolism, catabolic, and biosynthetic processes, ion transport, cell growth, and proliferation, were found to be different...Continue Reading

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Citations

Sep 26, 2017·Genome Biology and Evolution·Albin FontaineLouis Lambrechts
Nov 7, 2015·PLoS Neglected Tropical Diseases·Keshava MysoreMolly Duman-Scheel
Mar 15, 2021·Insect Molecular Biology·M McFarlaneE Pondeville
Jun 12, 2021·Journal of Evolutionary Biology·Carrie A WhittleCassandra G Extavour

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Datasets Mentioned

BETA
GPL18530
GSE56521
AAEL001123

Methods Mentioned

BETA
dissection
immunoprecipitation
MDS

Software Mentioned

DAVID
IDT
GenMAPP
Bioconductor
Blast
custom Perl script
Integrated DNA Technology ( IDT )
NimbleScan
FIJI
GenMAPP2

Related Concepts

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