Examination of the superoxide/hydrogen peroxide forming and quenching potential of mouse liver mitochondria.

Biochimica Et Biophysica Acta. General Subjects
Liam SladeRyan J Mailloux

Abstract

Pyruvate dehydrogenase (PDHC) and α-ketoglutarate dehydrogenase complex (KGDHC) are important sources of reactive oxygen species (ROS). In addition, it has been found that mitochondria can also serve as sinks for cellular hydrogen peroxide (H2O2). However, the ROS forming and quenching capacity of liver mitochondria has never been thoroughly examined. Here, we show that mouse liver mitochondria use catalase, glutathione (GSH), and peroxiredoxin (PRX) systems to quench ROS. Incubation of mitochondria with catalase inhibitor 3-amino-1,2,4-triazole (triazole) induced a significant increase in pyruvate or α-ketoglutarate driven O2-/H2O2 formation. 1-Choro-2,4-dinitrobenzene (CDNB), which depletes glutathione (GSH), elicited a similar effect. Auranofin (AF), a thioredoxin reductase-2 (TR2) inhibitor which disables the PRX system, did not significantly change O2-/H2O2 formation. By contrast catalase, GSH, and PRX were all required to scavenging extramitochondrial H2O2. In this study, the ROS forming potential of PDHC, KGDHC, Complex I, and Complex III was also profiled. Titration of mitochondria with 3-methyl-2-oxovaleric acid (KMV), a specific inhibitor for O2-/H2O2 production by KGDHC, induced a ~86% and ~84% decrease in ROS produc...Continue Reading

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Citations

Jan 21, 2018·Antioxidants & Redox Signaling·Petr JežekMartin Jabůrek
Jun 6, 2020·Antioxidants·Ryan J Mailloux
Feb 13, 2020·Oxidative Medicine and Cellular Longevity·Shyh-Horng LinShu-Fang Wen
Jul 31, 2018·Oxidative Medicine and Cellular Longevity·Ryan J Mailloux
Feb 9, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Michael O IseiCollins Kamunde
Dec 12, 2019·Free Radical Biology & Medicine·Michael O Isei, Collins Kamunde
Dec 9, 2020·Journal of Alzheimer's Disease : JAD·Dona P W JayatungaRalph N Martins
Jun 20, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Michael O IseiCollins Kamunde
Aug 22, 2021·Antioxidants & Redox Signaling·Eftekhar Eftekharpour, Paul Fernyhough

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