Examination of the Use of Bacteriophage as an Additive and Determining Its Best Application Method to Control Listeria monocytogenes in a Cooked-Meat Model System

Frontiers in Microbiology
Hanie AhmadiS Balamurugan

Abstract

The study examined the efficacy of using bacteriophage as an additive in a cooked-meat model system to control growth of contaminating Listeria monocytogenes during subsequent storage. Studies were designed where Listeria bacteriophage A511 and L. monocytogenes introduced inside or on the surface of the cooked-meat to simulate different bacteriophage application and pathogen contamination scenarios. These scenarios include: (1) A511 and L. monocytogenes in meat; (2) A511 in meat, L. monocytogenes on surface; (3) L. monocytogenes in meat, A511 on surface; and (4) L. monocytogenes followed by A511 on meat surface. Real world bacteriophage application and pathogen contamination levels of 109 PFU/g and 103-4 CFU/g, respectively, were used. These meats were then vacuum packaged and stored at 4°C and changes in A511 titers and L. monocytogenes numbers were enumerated during the 28-day storage. Under the conditions tested, application of A511 directly on top of L. monocytogenes contaminating the surface of the meat was the only scenario where L. monocytogenes numbers were reduced to below detection limits and remained significantly lower than the controls for up to 20 days. Although A511 titers remained stable when applied as an addit...Continue Reading

References

Dec 1, 1994·International Journal of Food Microbiology·S QvistP Zeuthen
Sep 15, 1994·FEMS Microbiology Letters·Y S TarahovskyA A Khusainov
Aug 21, 2001·Journal of Food Protection·B LeverentzA Sulakvelidze
Dec 7, 2002·The International Journal of Biochemistry & Cell Biology·Fumio ArisakaMichael G Rossmann
Apr 18, 2003·The Biological Bulletin·J A Fuhrman, M Schwalbach
Nov 18, 2008·Applied and Environmental Microbiology·Susanne GuentherMartin J Loessner
Dec 11, 2008·Methods in Molecular Biology·Andrew M KropinskiRoger P Johnson
May 20, 1940·The Journal of General Physiology·M Delbrück
Jan 7, 2010·Journal of Food Protection·Kamlesh A Soni, Ramakrishna Nannapaneni
Jun 21, 2011·Bacteriophage·Stephen T Abedon
Oct 5, 2012·Advances in Food and Nutrition Research·Lubov Y BrovkoMansel W Griffiths
Jul 7, 2014·Colloids and Surfaces. B, Biointerfaces·Zeinab HosseinidoustNathalie Tufenkji
Apr 8, 2015·The ISME Journal·Jessica M LabontéRamunas Stepanauskas
Dec 21, 2017·Frontiers in Microbiology·Hanie AhmadiSampathkumar Balamurugan
Dec 21, 2018·Methods in Molecular Biology·Andrew Chibeu, Sampathkumar Balamurugan

❮ Previous
Next ❯

Citations

Nov 14, 2020·Current Opinion in Biotechnology·Michela Gambino, Lone Brøndsted
Apr 6, 2021·Critical Reviews in Microbiology·Avijit GoswamiRachit Agarwal

❮ Previous
Next ❯

Software Mentioned

Intralytix
Statistical Analysis Systems

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.