Examination of the variability in mixed DNA profile parameters for the Identifiler multiplex

Forensic Science International. Genetics
Jo-Anne BrightJohn Buckleton

Abstract

The interpretation of mixed DNA profiles is often undertaken by determining to what extent possible genotype combinations may be eliminated from consideration. This requires an understanding of the variability in height or area for the two alleles of a heterozygote (heterozygote balance), and in the variability of mixture proportion or mixture ratio across loci. We present here empirical data to help assess the magnitude of this variability for Identifiler profiles. We find that heterozygote balance is affected by peak height as expected and that at heights above 267RFU the two peaks of a heterozygote demonstrates a peak height ratio between 0.6 and 1.66. For mixtures we introduce a concept, APH, the average peak heights of the active alleles. Above an APH of 110RFU mixture proportion demonstrated variation no more than 0.2 from the average across loci. Mixture ratio variability was affected by both the mixture ratio, M(r) per se and by the APH. The function (2APH/(1+M(r))) appears to be a reasonable predictor of the variability and approximates the expected peak height of the minor alleles. At (2APH/(1+M(r)))> or =300RFU the mixture ratio at a locus is expected to be within a factor of 2 of the average across loci.

References

Mar 11, 1998·Forensic Science International : Synergy·T M ClaytonP Gill
Jun 6, 2006·Forensic Science International : Synergy·P GillUNKNOWN DNA commission of the International Society of Forensic Genetics

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Citations

Dec 10, 2013·Forensic Science International. Genetics·Jose María AznarMarian M de Pancorbo
Dec 3, 2010·Investigative Genetics·Roland Ah van OorschotR John Mitchell
May 14, 2014·International Journal of Molecular Medicine·Na HuChunling Ma
May 7, 2014·Forensic Science International. Genetics·Mark D TimkenMartin R Buoncristiani
Jan 17, 2016·Forensic Science International. Genetics·Duncan TaylorJo-Anne Bright
Dec 27, 2015·Forensic Science International. Genetics·Duncan TaylorJohn Buckleton
Jul 17, 2012·Forensic Science International. Genetics·Christine A RakayCatherine M Grgicak
Apr 5, 2011·Forensic Science International. Genetics·Torben TvedebrinkNiels Morling
May 12, 2010·Forensic Science International. Genetics·Peter Gill, John Buckleton
Dec 9, 2014·Forensic Science International. Genetics·Hinda HanedTitia Sijen
Dec 30, 2014·Forensic Science International. Genetics·Stefano VernarecciPaola Montagna
May 21, 2014·Forensic Science International. Genetics·Kirk E LohmuellerKeith Inman
Dec 3, 2014·Forensic Science International. Genetics·Sarah L FordyceNiels Morling
Nov 4, 2016·International Journal of Legal Medicine·Oskar HanssonPeter Gill
May 16, 2017·Forensic Science International. Genetics·Tamyra R MorettiAnthony J Onorato
Oct 5, 2015·Forensic Science International. Genetics·Duncan TaylorJohn Buckleton
May 3, 2011·Analytical Chemistry·T A BrettellJ R Almirall

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