Examination of the xanthosine response on gene expression of mammary epithelial cells using RNA-seq technology

Journal of Animal Science and Technology
Shanti ChoudharyRatan K Choudhary

Abstract

Xanthosine treatment has been previously reported to increase mammary stem cell population and milk production in cattle and goats. However, the underlying molecular mechanisms associated with the increase in stem cell population and milk production remain unclear. Primiparous Beetal goats were assigned to the study. Five days post-partum, one mammary gland of each goat was infused with xanthosine (TRT) twice daily (2×) for 3 days consecutively, and the other gland served as a control (CON). Milk samples from the TRT and CON glands were collected on the 10th day after the last xanthosine infusion and the total RNA was isolated from milk fat globules (MEGs). Total RNA in MFGs was mainly derived from the milk epithelial cells (MECs) as evidenced by expression of milk synthesis genes. Significant differentially expressed genes (DEGs) were subjected to Gene Ontology (GO) terms using PANTHER and gene networks were generated using STRING db. Preliminary analysis indicated that each individual goat responded to xanthosine treatment differently, with this trend being correlated with specific DEGs within the same animal's mammary gland. Several pathways are impacted by these DEGs, including cell communication, cell proliferation and ant...Continue Reading

References

Jun 15, 1995·European Journal of Biochemistry·V L SpitsbergR C Gorewit
Sep 10, 2002·Reproduction, Nutrition, Development·Marion Boutinaud, Hélène Jammes
Jun 30, 2004·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Nicola Orsi
Apr 19, 2007·Cell and Tissue Research·Mark D CreganPeter E Hartmann
Nov 20, 2008·Physiological Genomics·Patricia D ManingatMorey W Haymond
Jan 30, 2009·Experimental Biology and Medicine·Anthony V CapucoDavid L Wood
Dec 2, 2009·Cell Adhesion & Migration·Mei Chung Moh, Shali Shen
Jun 16, 2012·BMC Cell Biology·Ratan K Choudhary, Anthony V Capuco
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Dec 18, 2013·BMC Genomics·Danielle G LemayJ Bruce German
Aug 26, 2014·Nature Biotechnology·Davide RissoSandrine Dudoit
Sep 13, 2014·In Vitro Cellular & Developmental Biology. Animal·Sonja Prpar MihevcPeter Dovc
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Apr 2, 2016·The Journal of Dairy Research·Qiuming ChenHesheng Jiang
Jun 10, 2016·International Journal of Oncology·Xuefeng ZhangQianming Chen

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA389156

Methods Mentioned

BETA
biopsy
PCR
electrophoresis
RNA-seq
GTPase
RNAseq
transgenic

Software Mentioned

RUVseq
STAR
PANTHER
Deseq2
STRING
Fiji
- BLAST
cuffdiff
Qubit
Primer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.