PMID: 9428697Jan 15, 1998Paper

Examining F-actin interaction with intact talin and talin head and tail fragment using static and dynamic light scattering

European Journal of Biochemistry
W H GoldmannG Isenberg

Abstract

We examined the binding kinetics of intact talin and talin head and tail fragment with F-actin at pH 7.0 and at low ionic strength. We observed by a transient kinetic method a fast followed by a slower binding process for intact talin and talin tail fragment with filamentous actin. The latter can be attributed to F-actin cross-linking and/or bundling, which was observed in cosedimentation assays as well as by low shear viscometry and electron microscopy [Zhang, J., Robson, R. M., Schmidt, J. M. & Stromer, M. H. (1996) Biochem. Biophys. Res. Commun. 218, 530-537]. This finding is supported by dynamic light scattering measurements, indicating changes in internal actin filament dynamics due to cross-linking/bundling events with intact talin and talin tail fragment. No binding of the talin head fragment with F-actin was detected by either method.

References

Jun 24, 1991·FEBS Letters·S KaufmannG Isenberg
Jul 31, 1991·Biochemical and Biophysical Research Communications·W H Goldmann, G Isenberg
May 1, 1990·The Journal of Cell Biology·G H NuckollsK Burridge
Oct 18, 1990·Nature·D J ReesR O Hynes
Sep 28, 1990·Biochemical and Biophysical Research Communications·M MugurumaT Fukazawa
Apr 19, 1984·Nature·K Burridge, P Mangeat
Sep 15, 1994·European Journal of Biochemistry·V NiggliG Isenberg
Jan 1, 1994·Journal of Structural Biology·W H GoldmannG Isenberg
Feb 1, 1994·Biochemical Society Transactions·W H GoldmannG Isenberg
Dec 15, 1993·Biochemical and Biophysical Research Communications·J M SchmidtM H Stromer
Jan 17, 1996·Biochemical and Biophysical Research Communications·J ZhangM H Stromer
May 1, 1995·Journal of Biochemistry·M MugurumaS Matsumura
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·R O McCann, S W Craig

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Citations

Aug 3, 2007·Molecular Biology of the Cell·Shawn A GaldeenMargaret A Titus
Jun 9, 2015·Nature Cell Biology·Lindsay B CaseClare M Waterman
Jul 19, 2013·Biochimica Et Biophysica Acta·Vasco SequeiraJolanda van der Velden
May 21, 1999·Archives of Biochemistry and Biophysics·J M SchmidtR M Robson

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