Examining the efficacy of intravenous administration of predatory bacteria in rats

Scientific Reports
Kenneth ShatzkesNancy D Connell

Abstract

The proteobacteria Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are obligate predators of Gram-negative bacteria, and have been proposed to be used to treat multidrug-resistant bacterial infections. The ability of predatory bacteria to reduce bacterial burden in vivo within the lungs of rats has been demonstrated, but it was unknown if predatory bacteria can attenuate systemic bacterial burden administered intravenously. In this study, we first assessed the safety of intravenous inoculation of predatory bacteria in rats. No rat morbidity or adverse histopathology of various organs due to predatory bacteria administration was observed. An increase in proinflammatory cytokines (TNFα and KC/GRO) was observed at two hours post-inoculation; however, cytokines returned to baseline levels by 18 hours. Furthermore, bacterial dissemination analysis demonstrated that predatory bacteria were efficiently cleared from the host by 20 days post-injection. To determine whether predatory bacteria could reduce bacterial burden in vivo, Klebsiella pneumoniae was injected into the tail veins of rats and followed with multiple doses of predatory bacteria over 16 or 24 hours. Predatory bacteria were unable to significantly reduce K. pne...Continue Reading

References

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Citations

Jul 25, 2017·Microbial Biotechnology·Harald Brüssow
Mar 5, 2019·Journal of Periodontology·Pedro H F SilvaMichel R Messora
May 12, 2019·Scientific Reports·James S FindlayPetra C F Oyston
Nov 14, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jyoti Madhusoodanan
Oct 10, 2020·Computational and Structural Biotechnology Journal·Juana PérezJosé Muñoz-Dorado
Jan 20, 2021·Microbiology·Robert J Atterbury, Jess Tyson

Methods Mentioned

BETA
enzyme linked-immunosorbent assays
PCR
ELISA

Related Concepts

Metazoa
Bacterial Infections
Enzyme-Linked Immunosorbent Assay
Morbidity - Disease Rate
Rats, Laboratory
Host-Pathogen Interactions
Klebsiella pneumoniae
Blood
Gram-Negative Bacteria
Lung

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