Excavating chikungunya genome to design B and T cell multi-epitope subunit vaccine using comprehensive immunoinformatics approach to control chikungunya infection

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Aruna NarulaVijay Kumar Prajapati

Abstract

Chikungunya infection has been a cause of countless deaths worldwide. Due to lack of permanent treatment and prevention of this disease, the mortality rate remains very high. Therefore, we followed an immunoinformatics approach for the development of multi-epitope subunit vaccine which is able to elucidate humoral, cell-mediated and innate immune responses inside the host body. Both structural and non-structural proteins of chikungunya virus were utilized for prediction of B-cell and T-cell binding epitopes along with interferon-γ (IFN-γ) inducing epitopes. The vaccine construct is composed of β-defensin as an adjuvant at the N-terminal followed by Cytotoxic T-Lymphocytes (CTL) and Helper T-Lymphocyte (HTL) epitopes. The same vaccine construct was also utilized for the prediction of B-cell binding epitopes and IFN-γ inducing epitopes. This was followed by the 3D model generation, refinement and validation of the vaccine construct. Later on, the interaction of modeled vaccine with the innate immune receptor (TLR-3) was explored by performing molecular docking and molecular dynamics simulation studies. Also to check the efficiency of expression of this vaccine construct in an expression vector, in silico cloning was performed at ...Continue Reading

Citations

Jul 27, 2018·Journal of Biomolecular Structure & Dynamics·Nazia KhatoonVijay Kumar Prajapati
Aug 3, 2019·Journal of Biomolecular Structure & Dynamics·Aziz Ul-Rahman, Muhammad Abu Bakr Shabbir
Dec 20, 2019·Journal of Biomolecular Structure & Dynamics·Stephane Fraga de Oliveira TostaLuiz Carlos Junior Alcantara
Sep 24, 2019·Human Vaccines & Immunotherapeutics· SunitaPratyoosh Shukla
Jan 19, 2019·PeerJ·Yao LeiYongguang Zhang
Sep 9, 2020·Journal of Biomolecular Structure & Dynamics·Daya V PavitrakarPratip Shil
Dec 1, 2020·Journal of Biomolecular Structure & Dynamics·Vijaya Sai AyyagariKrupanidhi Srirama
Nov 13, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Nabarun Chandra DasSuprabhat Mukherjee
Sep 12, 2020·Microbial Pathogenesis·MohammadReza Chaechi NosratiFarzaneh Shakarami
Mar 31, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Maria K SilvaJonas I N Oliveira
Apr 27, 2021·Informatics in Medicine Unlocked·Md Tahsin KhanMohammad A Halim

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