Excavatolide B inhibits nonsmall cell lung cancer proliferation by altering peroxisome proliferator activated receptor gamma expression and PTEN/AKT/NF-Kβ expression

Environmental Toxicology
Bharath Kumar VelmuruganChing-Feng Weng

Abstract

Marine organisms are proven to be rich source of secondary metabolites that can be used to treat various diseases. Excavatolide B (Exc.B), the most abundant metabolite was found in the marine coral Briareum excavatum exhibits cytotoxic effects against lung cancer cell. Treatment of the A549 cells with Exc.B significantly reduced its cell viability and induced cell cycle arrest at subG1 phase in a dose- and time-dependent manner, respectively. Apoptosis induction by Exc.B was further confirmed by decreased pro-caspase 3 expressions and increased proteolytic cleavage of poly (ADP-ribose) polymerase (PARP) expression. Furthermore, Exc.B increased reactive oxygen species (ROS) and reactive nitrogen species (RNS) and also decreased the antioxidant enzymes such as, Catalase, GPx, SOD, GST, and GSH. The proteomic analysis data revealed that total thirty six proteins were altered by Exc.B. STRING database showed that most of the altered proteins have no interaction between each other. Based on these data, KSR1, RuVBL2, PPAR-γ, and Tenascin X proteins were chosen to validate the 2DE data by Western blotting. Additional experiments demonstrated that Exc.B induced PTEN expression and inhibited pAKT and NF-kB expression. These results prov...Continue Reading

References

Sep 30, 1991·The American Journal of Medicine·H Sies
Jan 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L J Ignarro
Jan 16, 1995·FEBS Letters·S ToyokuniH Hiai
Oct 1, 1996·Journal of Natural Products·J H SheuT J Lee
Feb 5, 1998·CA: a Cancer Journal for Clinicians·S H LandisP A Wingo
Aug 28, 1998·European Journal of Pharmacology·B BrüneK B Sandau
Feb 29, 2000·Cell·A Di Cristofano, P P Pandolfi
Sep 29, 2001·Nature Immunology·C Bogdan
Feb 19, 2002·Genes & Development·François RoyMarc Therrien
Mar 5, 2002·Nature Immunology·Michael Karin, Anning Lin
Mar 14, 2002·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Hidefumi SasakiYoshitaka Fujii
Jan 22, 2003·Biochemical and Biophysical Research Communications·Buckminster Farrow, B Mark Evers
Mar 5, 2003·Journal of Cellular Biochemistry·Narayan ShivapurkarAdi F Gazdar
Jul 26, 2003·Journal of Natural Products·David J NewmanKenneth M Snader
Oct 29, 2003·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M L AmadorM Hidalgo
Dec 23, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lily L WuJames T Wu
Jan 20, 2004·Molecular and Cellular Biology·Krishna Murthi VasudevanVivek M Rangnekar
Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Ruth Chiquet-Ehrismann
Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Ruth Chiquet-Ehrismann, Richard P Tucker
Aug 31, 2004·Journal of Natural Products·David J Newman, Gordon M Cragg
Apr 28, 2005·Virchows Archiv : an International Journal of Pathology·Xavier DolcetXavier Matias-Guiu
Jan 3, 2006·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Sung Yong LeeSe Hwa Yoo
Feb 14, 2006·European Journal of Pharmacology·Henry Wing-Cheung LeungHong-Zin Lee
Mar 23, 2007·Nature Reviews. Molecular Cell Biology·Stefan J Riedl, Guy S Salvesen
Jul 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Benoît RousseauJean Rosenbaum
Sep 28, 2007·Nature Clinical Practice. Oncology·Sara C ErridgeDavid Brewster
Jun 3, 2009·Natural Product Research·V Lakshmi, R Kumar
Jul 21, 2009·Cancer Letters·Soon Young ShinYoongho Lim
Jan 12, 2010·Cellular Signalling·Nayana Ghosh-ChoudhuryGoutam Ghosh Choudhury
May 1, 2010·Planta medica·Kandan Aravindaram, Ning-Sun Yang
May 27, 2010·Journal of Natural Products·Shi-Yie ChengChang-Yih Duh

❮ Previous
Next ❯

Citations

Jan 22, 2019·Natural Product Reports·Anthony R CarrollMichèle R Prinsep
Mar 8, 2018·Journal of Experimental & Clinical Cancer Research : CR·Zhikui LiuKangsheng Tu
Feb 22, 2017·Marine Drugs·Yin-Di SuPing-Jyun Sung
Sep 9, 2017·Frontiers in Molecular Biosciences·Yu-Qian Mao, Walid A Houry
Aug 28, 2020·Oncotarget·Justyna Magdalena HermanowiczDariusz Pawlak
Jun 4, 2019·Frontiers in Oncology·Rafah A A AlnafakhDharani K Hapangama
Aug 10, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jiaen WeiZunnan Huang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Control & Proteolysis

Key regulators of cell cycle, including cyclins, cyclin dependent kinase inhibitors, DNA replication factors, are controlled by proteolysis. Discover the latest research on cell cycle control and proteolysis.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis