Excess androgen production in subcutaneous adipose tissue of women with polycystic ovarian syndrome is not related to insulin or LH

The Journal of Endocrinology
Saad Aks AmerRaheela Khan

Abstract

The purpose of this study was to investigate androgen production and the role of insulin and LH in its regulation in subcutaneous adipose tissue (SAT) of women with polycystic ovarian syndrome (PCOS). Protein and mRNA expression of androgen synthesis enzymes (Cytochrome P450 17A1 [CYP17A1] and Aldo-keto reductase 1C3 [AKR1C3]) were measured in SAT biopsies from women with PCOS, diagnosed according to the Rotterdam criteria (n=15) and healthy controls (n=15). Cultured mature adipocytes (differentiated from SAT biopsies) were treated with insulin ± phosphoinositol-3-kinase inhibitor (LY294002) or LH ± insulin. CYP17A1 and AKR1C3 mRNA expression and testosterone concentrations were measured in treated and untreated adipocyte cultures. AKR1C3 mRNA was significantly (P<0.001) greater in PCOS versus non-PCOS SAT, but CYP17A1 was not significantly different between the two groups. AKR1C3 and CYP17A1 protein expression was not significantly different in PCOS versus non-PCOS SAT. In untreated adipocyte cultures, CYP17A1, AKR1C3 and testosterone levels were significantly higher in the PCOS versus the non-PCOS groups. Addition of insulin increased AKR1C3 mRNA and testosterone levels, but not CYP17A1 mRNA in non-PCOS with no effect on PCOS...Continue Reading

References

Aug 1, 1992·The Journal of Clinical Endocrinology and Metabolism·T P CiaraldiS S Yen
Feb 1, 1990·The Journal of Clinical Endocrinology and Metabolism·M A KirschnerN Ertel
Dec 9, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·T Fehér, L Bodrogi
Nov 1, 1984·The Journal of Clinical Endocrinology and Metabolism·E SamojlikN H Ertel
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·L H ZhangW L Miller
Nov 5, 1999·Human Reproduction·E KoustaS Franks
Feb 9, 2002·European Journal of Endocrinology·Cristina PucheAnna Meseguer
Sep 4, 2003·Genome Biology·Dov GreenbaumMark Gerstein
Aug 25, 2004·Molecular & Cellular Proteomics : MCP·Andreas BeyerThomas Wilhelm
Oct 21, 2004·American Journal of Physiology. Endocrinology and Metabolism·Karine BlouinAndré Tchernof
Jun 10, 2005·Clinical Endocrinology·Ashim KumarRicardo Azziz
Aug 19, 2007·Trends in Endocrinology and Metabolism : TEM·Helena J TeedeSophia Zoungas
Nov 29, 2007·International Journal of Obesity : Journal of the International Association for the Study of Obesity·E Diamanti-Kandarakis
Apr 16, 2008·Clinical Endocrinology·Scott M MacKenzieEleanor Davies
Oct 24, 2009·FEBS Letters·Tobias MaierLuis Serrano
Mar 3, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Lihua WangWei Liu
Oct 16, 2012·Endocrine Reviews·Evanthia Diamanti-Kandarakis, Andrea Dunaif
Jun 29, 2014·Molecular Genetics and Metabolism Reports·David CadaganSaad Amer
Jun 25, 2017·The Journal of Clinical Endocrinology and Metabolism·Michael W O'ReillyWiebke Arlt
Jan 18, 2018·The Journal of Clinical Endocrinology and Metabolism·Yasir S ElhassanMichael W O'Reilly

❮ Previous
Next ❯

Citations

Jul 9, 2020·Therapeutic Advances in Endocrinology and Metabolism·Punith KempegowdaMichael W O'Reilly
Apr 4, 2021·International Journal of Molecular Sciences·Shaimaa AboeldalylSaad Amer
May 29, 2020·Journal of Medicinal Chemistry·Yang LiuHaopeng Sun

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.