Excess copper induces structural changes in cultured photosynthetic soybean cells

Functional Plant Biology : FPB
María BernalInmaculada Yruela

Abstract

Soybean [Glycine max (L.) Merr.] cell suspensions have the capacity to develop tolerance to excess copper, constituting a convenient system for studies on the mechanisms of copper tolerance. The functional cell organisation changes observed in these cell cultures after both short-term (stressed cells) and long-term (acclimated cells) exposure to 10 μm CuSO4 are reported from structural, cytochemical and microanalytical approaches. Cells grown in the presence of 10 μm CuSO4 shared some structural features with untreated cells, such as: (i) a large cytoplasmic vacuole, (ii) chloroplasts along the thin layer of cytoplasm, (iii) nucleus in a peripheral location exhibiting circular-shaped nucleolus and a decondensed chromatin pattern, and (iv) presence of Cajal bodies in the cell nuclei. In addition, cells exposed to 10 μm CuSO4 exhibited important differences compared with untreated cells: (i) chloroplasts displayed rounded shape and smaller size with denser-structured internal membranes, especially in copper-acclimated cells; (ii) no starch granules were found within chloroplasts; (iii) the cytoplasmic vacuole was larger, especially after long-term copper exposure; (iv) the levels of citrate and malate increased. Extracellular dar...Continue Reading

References

Jun 15, 1994·Microscopy Research and Technique·E PålsgårdG M Roomans
Jun 17, 1998·Free Radical Biology & Medicine·W Y Kuo, T K Tang
Jun 13, 2002·Physiologia Plantarum·Nathalie A. L. M. Van HoofWilfried H. O. Ernst
Oct 31, 2003·Journal of Experimental Botany·J L Hall, Lorraine E Williams
Jan 9, 2004·Applied and Environmental Microbiology·Asunción de Los RíosAntonio Quesada
Sep 21, 2004·Annual Review of Plant Biology·Leon V KochianMiguel A Pineros
Mar 9, 2005·Cytogenetic and Genome Research·P S TestillanoM C Risueño
Sep 20, 2005·Photosynthesis Research·Jotham Austin Ii, Andrew N Webber
Apr 18, 2006·Current Opinion in Plant Biology·Marinus PilonHong Ye
Dec 21, 2006·Plant Biotechnology Journal·Sylvia de PaterNick de Vetten
Jul 1, 2005·Functional Plant Biology : FPB·Priscila L GratãoRicardo A Azevedo
Feb 1, 2006·Functional Plant Biology : FPB·Andrew C AllanIan B Ferguson
Jul 1, 2006·Functional Plant Biology : FPB·Anna ZuppiniBarbara Baldan

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Methods Mentioned

BETA
light microscopy
X-ray
scanning electron microscopy

Software Mentioned

LTSEM
Quantax

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