Excess iodide decreases transcription of NIS and VEGF genes in rat FRTL-5 thyroid cells.

Biochemical and Biophysical Research Communications
Koichi SuzukiLeonard D Kohn

Abstract

Although it is well known that an excess of iodide suppresses thyroid function and blood flow in vivo, the underlying molecular mechanisms are not fully known. The functional effect of iodide occurs at multiple steps, which include inhibition of sodium/iodide symporter (NIS) expression, transient block of organification, and inhibition of hormonal release. The vascular effect likely involves suppression of the vascular endothelial growth factor (VEGF) gene. In this report, we show that excess iodide coordinately suppresses the expression of the NIS and VEGF genes in FRTL-5 thyroid cells. We also demonstrate that the mechanism of iodide suppression of NIS gene expression is transcriptional, which is synergized by the addition of thyroglobulin. Based on the findings of reporter gene assays and electrophoretic gel mobility shift analysis, we also report two novel DNA binding proteins that responded specifically to iodide and modulated NIS promoter activity. The results suggest that excess iodide affects thyroid vascular function in addition to iodide uptake. This study provides additional insights into the mechanism of action of excess iodide on thyroid function.

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Citations

Aug 3, 2012·Thyroid : Official Journal of the American Thyroid Association·Mariko SueKoichi Suzuki
Dec 14, 2012·Thyroid : Official Journal of the American Thyroid Association·Akira KawashimaKoichi Suzuki
Jun 17, 2014·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Bernhard Michalke, Heidi Witte
Feb 14, 2016·Molecular and Cellular Endocrinology·Caroline Serrano-NascimentoMaria Tereza Nunes
Apr 26, 2016·Experimental Cell Research·Madeleine Nordén LyckesvärdMikael Nilsson
Aug 29, 2016·Molecular and Cellular Endocrinology·Luciano E RossichGuillermo J Juvenal
Nov 7, 2016·General and Comparative Endocrinology·Alison M GardellC Loren Buck
Feb 6, 2019·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Chung-Hsin TsaiShih-Ping Cheng
Mar 8, 2019·Endocrine Journal·Sadegh RajabiMehdi Hedayati
Dec 21, 2016·Aging·Kayla Daniell, Carmelo Nucera

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