Excess vascular endothelial growth factor-A disrupts pericyte recruitment during blood vessel formation

Angiogenesis
Jordan DardenJohn C Chappell

Abstract

Pericyte investment into new blood vessels is essential for vascular development such that mis-regulation within this phase of vessel formation can contribute to numerous pathologies including arteriovenous and cerebrovascular malformations. It is critical therefore to illuminate how angiogenic signaling pathways intersect to regulate pericyte migration and investment. Here, we disrupted vascular endothelial growth factor-A (VEGF-A) signaling in ex vivo and in vitro models of sprouting angiogenesis, and found pericyte coverage to be compromised during VEGF-A perturbations. Pericytes had little to no expression of VEGF receptors, suggesting VEGF-A signaling defects affect endothelial cells directly but pericytes indirectly. Live imaging of ex vivo angiogenesis in mouse embryonic skin revealed limited pericyte migration during exposure to exogenous VEGF-A. During VEGF-A gain-of-function conditions, pericytes and endothelial cells displayed abnormal transcriptional changes within the platelet-derived growth factor-B (PDGF-B) and Notch pathways. To further test potential crosstalk between these pathways in pericytes, we stimulated embryonic pericytes with Notch ligands Delta-like 4 (Dll4) and Jagged-1 (Jag1) and found induction of ...Continue Reading

References

Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·S HiratsukaM Shibuya
May 2, 2001·The Journal of Cell Biology·M HellströmC Betsholtz
Jun 18, 2003·The Journal of Cell Biology·Holger GerhardtChrister Betsholtz
Jul 4, 2003·The Journal of Clinical Investigation·Shu-Ching ShihLois E H Smith
Jul 29, 2003·Cell and Tissue Research·Holger Gerhardt, Christer Betsholtz
Sep 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Erika IivanainenKlaus Elenius
Dec 31, 2003·Methods in Enzymology·Joseph B Kearney, Victoria L Bautch
Apr 27, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Martin HagedornAndreas Bikfalvi
Sep 17, 2005·Circulation Research·Annika ArmulikChrister Betsholtz
Oct 11, 2005·Neuro-oncology·Gabriele Bergers, Steven Song
May 20, 2006·Biochemical Society Transactions·S KurupD Spillmann
Jun 8, 2006·Journal of Biomedical Materials Research. Part a·Benjamin L BecksteadCecilia M Giachelli
Aug 19, 2007·Microvascular Research·Laura S HarringtonAdrian L Harris
Feb 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Frances A HighJonathan A Epstein
Mar 19, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Arndt F SiekmannNathan D Lawson
May 28, 2008·The Journal of Cell Biology·Nicholas C KappasVictoria L Bautch
Nov 11, 2008·Nature·Joshua I GreenbergDavid A Cheresh
Nov 26, 2008·Nature Reviews. Cancer·Christian FischerPeter Carmeliet
Jan 24, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Konstantin GaengelChrister Betsholtz
Feb 28, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Irina V LarinaMary E Dickinson
Aug 20, 2009·The Journal of Clinical Investigation·Ethan David CohenEdward E Morrisey
Sep 18, 2009·Developmental Cell·John C ChappellVictoria L Bautch
Oct 17, 2009·Stroke; a Journal of Cerebral Circulation·Gabrielle G LeblancUNKNOWN Biology of Vascular Malformations of the Brain NINDS Workshop Collaborators
Oct 27, 2009·Developmental Cell·Boris StrilićEckhard Lammert
Nov 17, 2009·Biochemical Society Transactions·Lars JakobssonHolger Gerhardt
Jan 2, 2010·Brain Research Reviews·Jacqueline TrotterAkiko Nishiyama
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Renhai CaoYihai Cao
Aug 10, 2010·Current Topics in Developmental Biology·John C Chappell, Victoria L Bautch
Aug 21, 2010·Frontiers in Neuroenergetics·Nicola B HamiltonCatherine N Hall
Oct 15, 2010·Nature·Annika ArmulikChrister Betsholtz

❮ Previous
Next ❯

Citations

May 9, 2019·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Laura B PayneJohn C Chappell
Jun 20, 2019·Journal of Cellular Physiology·Xiuling ShangRongguo Yu
Feb 7, 2020·Interventional Neuroradiology : Journal of Peritherapeutic Neuroradiology, Surgical Procedures and Related Neurosciences·Takahiro Ota, Masaki Komiyama
Mar 7, 2020·International Journal of Molecular Sciences·Micol MarchettiCostanza Emanueli
Aug 30, 2019·Journal of Receptor and Signal Transduction Research·Long BaoZhixiong Lin
May 23, 2020·International Journal of Cancer. Journal International Du Cancer·Qi HeMichael Welsh
Apr 16, 2019·Journal of Biological Engineering·Huaning Zhao, John C Chappell
Sep 11, 2019·Journal of Developmental Biology·John C ChappellVictoria L Bautch
Nov 11, 2020·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Maruf M HoqueJohn C Chappell
Jan 31, 2021·Integrative Biology : Quantitative Biosciences From Nano to Macro·Laura Beth PayneJohn C Chappell
Mar 23, 2021·Current Tissue Microenvironment Reports·Laura Beth PayneJohn C Chappell
Mar 30, 2021·Frontiers in Cellular Neuroscience·Hannah C Bennett, Yongsoo Kim
Apr 17, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Chengcheng SunHao Li
Jun 3, 2021·International Journal of Molecular Sciences·Mannon GeindreauMélanie Bruchard
May 30, 2021·Angiogenesis·Arjan W Griffioen, Andrew C Dudley
Apr 5, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhixiong MeiNi Deng
Apr 14, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lijuan ZhangHongyi Cai

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
dissection
fluorescence microscopy
Protein Assay
electrophoresis

Software Mentioned

ImageJ
FIJI
PrimerQuest
QuantStudio Expression qRT - PCR
LaserGene
Rad Image Lab
GraphPad Prism

Related Concepts

Related Feeds

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Alagille Syndrome

Alagille syndrome is a multi-system genetic disorder that can affect the liver, heart, and other parts of the body. It’s characterization includes heart problems, liver damage, jaundice, and xanthomas. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

International Journal of Cardiology
Christian G M van DijkCaroline Cheng
Frontiers in Cell and Developmental Biology
Emmanuel NwadoziTara L Haas
Microcirculation : the Official Journal of the Microcirculatory Society, Inc
Huaning ZhaoJohn C Chappell
© 2022 Meta ULC. All rights reserved