Excessive excretion of cyclic beta-(1,2)-glucan by Rhizobium trifolii TA-1.

Applied and Environmental Microbiology
M W BreedveldA J Zehnder

Abstract

At 25 degrees C, the optimal temperature for growth of Rhizobium trifolii TA-1, extracellular and capsular polysaccharide (EPS and CPS) were the main carbohydrate products synthesized in mannitol-rich medium (10 g of mannitol and 1 g of glutamic acid per liter). In the same medium at 33 degrees C, EPS and CPS production was inhibited, and up to 3.9 g of cyclic beta-(1,2)-glucan was produced during an incubation period of 20 days with a total biomass of 0.55 g of protein. In a medium containing 50 g of mannitol and 10 g of glutamic acid per liter, high cell densities (3.95 g of protein) were obtained at 25 degrees C. This biomass excreted 10.9 g of cyclic beta-(1,2)-glucan within 10 days. Concomitantly, 4.8 g of EPS were synthesized, while CPS production was strongly suppressed. The excreted cyclic beta-(1,2)-glucans were neutral and had degrees of polymerization ranging from 17 to 25, with a degree of polymerization of 19 as the major glucan cycle.

References

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Oct 1, 1985·Journal of Bacteriology·V PuvanesarajahE W Nester
Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen
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Citations

Jan 12, 2019·World Journal of Microbiology & Biotechnology·Puja KumariMukesh Doble
Mar 24, 2006·Molecular Microbiology·Marc C LausJan W Kijne
Feb 1, 1993·Journal of Bacteriology·M W BreedveldA J Zehnder
Mar 1, 1995·Applied and Environmental Microbiology·M W BreedveldK J Miller
Feb 1, 1992·Applied and Environmental Microbiology·J G StreeterR W Carlson
Jun 1, 1994·Microbiological Reviews·M W Breedveld, K J Miller
Mar 12, 2004·Carbohydrate Research·Sanghoo Lee, Seunho Jung
Aug 29, 2006·Research in Microbiology·Panlada TittabutrDulal Borthakur

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