Abstract
The propeptide of carboxypeptidase Y from Saccharomyces cerevisiae is important for folding of the enzyme. Previous work [Ramos, C., Winther, J.R. & Kielland-Brandt, M. C. (1994) J. Biol. Chem. 269, 7006-7012] suggested that the sequences essential for in vivo folding were situated in the COOH-proximal third of the propeptide. Concentrating on this region we have investigated the functionality of propeptide variants. Using a random mutagenesis approach we found that two segments can be defined: one in which there is a fairly high tolerance for substitution with unrelated sequences and another that has a more strict requirement for sequence conservation. Nevertheless, an overall lack of requirement for propeptide sequence conservation was found by substitution of the carboxypeptidase Y propeptide with that of a highly divergent propeptide sequence from an otherwise similar carboxypeptidase from Candida albicans. This propeptide was partially functional when combined with carboxypeptidase Y. Analysis of the biosynthesis of the mutant forms of the zymogen showed that a fraction of the molecules proceeded from the endoplasmic reticulum with fairly rapid kinetics, while the rest was degraded.
References
Apr 17, 1978·European Journal of Biochemistry·A Hasilik, W Tanner
Nov 2, 1992·Gene·M MukhtarN F Käufer
Mar 19, 1992·Nature·D BakerD A Agard
Feb 12, 1991·Biochemistry·J BuchnerT Kiefhaber
Jan 2, 1992·Nature·M J Gething, J Sambrook
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J R Winther, P Sørensen
Jan 1, 1991·Methods in Enzymology·F Sherman
May 8, 1991·European Journal of Biochemistry·J R WintherM C Kielland-Brandt
Aug 1, 1990·The Journal of Cell Biology·L A VallsT H Stevens
May 1, 1989·Genetics·R S Sikorski, P Hieter
Jul 1, 1986·Molecular and Cellular Biology·G AmmererT H Stevens
Mar 13, 1987·Cell·L A VallsT H Stevens
May 1, 1988·Molecular and Cellular Biology·D J KlionskyS D Emr
May 1, 1986·The Journal of Cell Biology·T H StevensR Schekman
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Sep 1, 1982·Cell·T StevensR Schekman
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
May 1, 1994·Trends in Biochemical Sciences·U Jakob, J Buchner
Dec 1, 1993·Current Opinion in Cell Biology·D BakerD A Agard
May 20, 1994·Cell·E G MarcussonS D Emr
Aug 17, 1993·Biochemistry·S StrausbergP Bryan
Apr 1, 1993·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·A R Tolat, J K Stanley
Jan 12, 1996·The Journal of Biological Chemistry·F van VoorstJ R Winther
Jan 1, 1996·Yeast·H B Van Den HazelJ R Winther
Citations
Apr 6, 2005·Functional & Integrative Genomics·Ying Feng, Qingzhong Xue
Aug 19, 2000·The Plant Cell·C LehfeldtC Chapple
May 15, 2015·PloS One·Atsushi SatomuraMitsuyoshi Ueda
Oct 1, 2010·Biomolecular Concepts·Ilya V DemidyukSergey V Kostrov
Jul 11, 1998·Journal of Bacteriology·S S IngavaleA K Bachhawat