PMID: 8954149Nov 15, 1996Paper

Exchange of regions of the carboxypeptidase Y propeptide. Sequence specificity and function in folding in vivo

European Journal of Biochemistry
C Ramos, J R Winther

Abstract

The propeptide of carboxypeptidase Y from Saccharomyces cerevisiae is important for folding of the enzyme. Previous work [Ramos, C., Winther, J.R. & Kielland-Brandt, M. C. (1994) J. Biol. Chem. 269, 7006-7012] suggested that the sequences essential for in vivo folding were situated in the COOH-proximal third of the propeptide. Concentrating on this region we have investigated the functionality of propeptide variants. Using a random mutagenesis approach we found that two segments can be defined: one in which there is a fairly high tolerance for substitution with unrelated sequences and another that has a more strict requirement for sequence conservation. Nevertheless, an overall lack of requirement for propeptide sequence conservation was found by substitution of the carboxypeptidase Y propeptide with that of a highly divergent propeptide sequence from an otherwise similar carboxypeptidase from Candida albicans. This propeptide was partially functional when combined with carboxypeptidase Y. Analysis of the biosynthesis of the mutant forms of the zymogen showed that a fraction of the molecules proceeded from the endoplasmic reticulum with fairly rapid kinetics, while the rest was degraded.

References

Mar 19, 1992·Nature·D BakerD A Agard
Jan 2, 1992·Nature·M J Gething, J Sambrook
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J R Winther, P Sørensen
Jan 1, 1991·Methods in Enzymology·F Sherman
Aug 1, 1990·The Journal of Cell Biology·L A VallsT H Stevens
May 1, 1986·The Journal of Cell Biology·T H StevensR Schekman
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
May 1, 1994·Trends in Biochemical Sciences·U Jakob, J Buchner
Dec 1, 1993·Current Opinion in Cell Biology·D BakerD A Agard
Apr 1, 1993·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·A R Tolat, J K Stanley
Jan 1, 1996·Yeast·H B Van Den HazelJ R Winther

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Citations

Apr 6, 2005·Functional & Integrative Genomics·Ying Feng, Qingzhong Xue
Oct 1, 2010·Biomolecular Concepts·Ilya V DemidyukSergey V Kostrov

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