Exchange of substrate and inhibitor specificities between adenylyl and guanylyl cyclases.

The Journal of Biological Chemistry
R K SunaharaA G Gilman

Abstract

The active sites of guanylyl and adenylyl cyclases are closely related. The crystal structure of adenylyl cyclase and modeling studies suggest that specificity for ATP or GTP is dictated in part by a few amino acid residues, invariant in each family, that interact with the purine ring of the substrate. By exchanging these residues between guanylyl cyclase and adenylyl cyclase, we can completely change the nucleotide specificity of guanylyl cyclase and convert adenylyl cyclase into a nonselective purine nucleotide cyclase. The activities of these mutant enzymes remain fully responsive to their respective stimulators, sodium nitroprusside and Gsalpha. The specificity of nucleotide inhibitors of guanylyl and adenylyl cyclases that do not act competitively with respect to substrate are similarly altered, indicative of their action at the active sites of these enzymes.

References

Jan 15, 1991·Biochemical and Biophysical Research Communications·W A BuechlerF Murad
Jan 1, 1991·Methods in Enzymology·S E DominoD L Garbers
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jul 1, 1990·Acta Crystallographica. Section A, Foundations of Crystallography·A T BrüngerJ W Erickson
Nov 7, 1995·Biochemistry·W J TangA G Gilman
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·B WedelD Koesling
Jan 1, 1994·Molecular Biology of the Cell·D L GarbersG Schultz
Dec 9, 1994·The Journal of Biological Chemistry·D L Garbers, D G Lowe
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·R E WhisnantC W Dessauer
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·R K SunaharaA G Gilman
Feb 11, 1997·Biochemistry·A FriebeD Koesling
Mar 20, 1997·Nature·G ZhangJ H Hurley
Aug 29, 1997·The Journal of Biological Chemistry·R K SunaharaA G Gilman
Aug 29, 1997·The Journal of Biological Chemistry·C W DessauerA G Gilman
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y LiuJ H Hurley
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren

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Citations

Apr 9, 2002·Journal of Biosciences·Avinash R ShenoySandhya S Visweswariah
Jun 14, 2008·Journal of Molecular Evolution·Jinyu WuFangqing Zhao
Jun 6, 2009·Journal of Molecular Evolution·Kabir Hassan BiswasSandhya S Visweswariah
Nov 8, 2001·European Journal of Pharmacology·M Rabbani, B Tabakoff
Jun 4, 1999·Trends in Pharmacological Sciences·C W DessauerA G Gilman
Dec 20, 2000·General Pharmacology·S Andreopoulos, A Papapetropoulos
Mar 24, 2004·Brain Research Bulletin·Joshua S KrumenackerFerid Murad
Oct 25, 2003·Cellular Signalling·Jürgen U Linder, Joachim E Schultz
Jan 23, 1999·Current Opinion in Structural Biology·J J Tesmer, S R Sprang
Jan 23, 1999·Current Opinion in Structural Biology·J H Hurley
Feb 27, 2009·Biochemistry·Tung-Chung MouStephen R Sprang
Jun 17, 2009·Biochemistry·Emily R DerbyshireMichael A Marletta
Sep 21, 2012·Journal of Medicinal Chemistry·Keith ó ProinsiasDorota Gryko
Sep 7, 2006·Nature Reviews. Drug Discovery·Oleg V EvgenovJohannes-Peter Stasch
Apr 2, 2009·Nature Reviews. Drug Discovery·Sandra PierreKlaus Scholich
Mar 10, 2001·The Biochemical Journal·J RoelofsP J Van Haastert
Oct 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Annika RauchClemens Steegborn
Jun 3, 2008·The Journal of Biological Chemistry·Xiaohui HuWilliam R Montfort
May 23, 2006·Assay and Drug Development Technologies·Sabrina CorazzaChiara Liberati
Apr 25, 2014·Journal of Caffeine Research·Sergi FerréXavier Guitart
Jul 12, 2002·The EMBO Journal·Tobias KanacherJoachim E Schultz
Dec 16, 2011·The Journal of Pharmacology and Experimental Therapeutics·Iraida SharinaEmil Martin
Sep 6, 2012·Science Signaling·Roland Seifert, Kerstin Y Beste
Jun 13, 2000·Journal of Bacteriology·J A Ochoa De AldaJ Houmard
Jun 9, 2000·Annual Review of Neuroscience·A D Gibson, D L Garbers
Feb 22, 2001·Annual Review of Physiology·B Wedel, D Garbers
Aug 18, 2012·Investigative Ophthalmology & Visual Science·Jianfeng WangEllen Townes-Anderson
Oct 10, 2008·BMC Structural Biology·Jonathan A WingerJohn Kuriyan
Nov 6, 2008·Journal of Biology·John J G Tesmer
Mar 19, 2013·PloS One·Charles K AllerstonOpher Gileadi
Aug 6, 2005·Zoological Science·Takumi Shiga, Norio Suzuki
Jun 5, 2014·World Journal of Surgical Oncology·Jia ZhangJiansheng Wang
Apr 12, 2014·Plant Physiology and Biochemistry : PPB·Brygida SwieżawskaAdriana Szmidt-Jaworska
Mar 13, 2012·Annual Review of Biochemistry·Emily R Derbyshire, Michael A Marletta
Dec 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·I Ruiz-StewartS A Waldman
Aug 6, 2011·Canadian Journal of Physiology and Pharmacology·Kailash N Pandey

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