PMID: 9661299Jul 14, 1998Paper

Exchangeability of actin in cardiac myocytes and fibroblasts as determined by fluorescence photobleaching recovery

Tissue & Cell
H SuzukiY Shimada

Abstract

Rhodamine (Rho)-labeled muscle and non-muscle actins were microinjected into cultured embryonic chicken cardiac myocytes and fibroblasts. After incorporation of the fluorescent actin analog into cellular structures, small areas of labeled structures were photobleached with a laser pulse, and fluorescence recovery (FR) was measured to determine the exchangeability of isoactins in these structures. With both Rho-muscle and Rho-non-muscle actins, the FR rate in any part of stress fibers was consistently faster than that observed in any part of myofibrils. Thus, although non-striated (proximal and terminal) portions of nascent myofibrils are similar in appearance and composition to stress fibers, our data clearly revealed differences in actin stability between these two structures. Further, although cardiomyocytes were incapable of discriminating between the incorporation of muscle and non-muscle actin isoforms into myofibrils, FR after photobleaching of Rho-muscle actin was faster than that of Rho-non-muscle actin in immature non-striated portions. This indicates that actin molecules in cardiac myofibrils cannot be readily exchanged by heterotypic non-muscle actin. Fluorescently labeled actin incorporated into non-striated (proxim...Continue Reading

References

Jun 1, 1986·The Journal of Cell Biology·J M SangerJ W Sanger
Mar 1, 1988·Cell Differentiation·J S DomeJ W Sanger
Mar 1, 1986·The Journal of Cell Biology·P A Amato, D L Taylor
Dec 1, 1985·The Journal of Cell Biology·N M McKennaY L Wang
Dec 1, 1984·The Journal of Cell Biology·W M SaxtonJ R McIntosh
Feb 1, 1997·Cell Structure and Function·Y ShimadaA Konno

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Citations

Sep 8, 2012·Cellular and Molecular Life Sciences : CMLS·Jennifer England, Siobhan Loughna
Mar 13, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Michele A JaegerJames M Ervasti
May 11, 2011·The Journal of Cell Biology·Katharina da Silva LopesMichael Gotthardt
Sep 15, 2005·The Journal of Cell Biology·Abigail S McElhinnyCarol C Gregorio
Mar 26, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Elisabeth EhlerJean-Claude Perriard
Jun 18, 2010·European Journal of Cell Biology·Anja LinnemannDieter O Fürst
Jun 5, 2001·Nature Cell Biology·R LittlefieldV M Fowler
May 6, 2010·The Journal of Biological Chemistry·Kuo-Kuang WenPeter A Rubenstein
May 28, 2009·Journal of Cell Science·Aneta Skwarek-MaruszewskaPekka Lappalainen

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