PMID: 9161037Apr 1, 1997Paper

Exchanging sequence domains between S-RNases from Nicotiana alata disrupts pollen recognition

The Plant Journal : for Cell and Molecular Biology
D. M. ZurekB. A. McClure

Abstract

In self-incompatible plants of the Solanaceae, the specificity of pollen rejection is controlled by a single multiallelic S-locus. Pollen tube growth is inhibited in the style when its single S-allele matches either S-allele present in the diploid pistil. Each S-allele encodes an S-RNase with a unique sequence. S-RNases are secreted into the extra-cellular matrix of the transmitting tract which guides pollen tubes toward the ovary. Although it is known that S-RNases are the determinants of S-allele specificity in the pistil, it is not known how allele-specific information is encoded in the sequence. Therefore, we exchanged domains between S-RNases with different recognition specificities and expressed the chimeric proteins in transgenic plants to determine their effects on pollination behavior. Nine chimeric constructs were prepared in which domains from Nicotiana alata SA2- and SC10-RNases were exchanged. Among these nine constructs, the entire S-RNase sequence was sampled by exchanging single variable domains as well as larger blocks of contiguous sequences. The chimeric S-RNases retained enzymatic activity and were expressed at levels comparable to control transformants expressing SA2- and SC10-RNases. However, none of the c...Continue Reading

Citations

Jun 2, 2007·Plant Cell Reports·Hiroyuki KakuiHidenori Sassa
Nov 9, 2007·Molecular Genetics and Genomics : MGG·Bruce G SutherlandTimothy P Robbins
Nov 15, 2003·Molecular Phylogenetics and Evolution·Eric H Roalson, Andrew G McCubbin
Mar 6, 1999·Current Opinion in Plant Biology·S McCormick
Feb 27, 1999·Current Opinion in Plant Biology·L K Wilhelmi, D Preuss
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·B McClureR Bernatzky
Nov 29, 2002·Journal of Experimental Botany·Yan WangTeh-Hui Kao
Nov 29, 2002·Journal of Experimental Botany·Felipe Cruz-GarciaBruce McClure
Jul 2, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C Nathan HancockBruce McClure
Mar 11, 2004·The Plant Cell·Teh-hui Kao, Tatsuya Tsukamoto
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A G McCubbin, T Kao
Aug 23, 2011·BMC Evolutionary Biology·Timothy Paape, Joshua R Kohn
Mar 31, 2001·Genes & Genetic Systems·M K Uyenoyama
Aug 2, 2011·Annals of Botany·Bruce McClureCarlos Romero
Jan 5, 2011·Annals of Botany·Xiaoying MengTeh-hui Kao
Dec 17, 2014·Molecular Genetics and Genomics : MGG·Lauís Brisolara-CorrêaLoreta Brandão de Freitas
Dec 16, 2005·The Journal of Biological Chemistry·Javier Andrés Juárez-DíazFelipe Cruz-García
Apr 18, 2021·The New Phytologist·Amanda K BrozPatricia A Bedinger
Oct 19, 2001·Genetics·C MiegeJ M Cock
May 4, 1999·Genetics·P Awadalla, D Charlesworth

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