Exchanging the yellow fever virus envelope proteins with Modoc virus prM and E proteins results in a chimeric virus that is neuroinvasive in SCID mice

Journal of Virology
Nathalie CharlierJohan Neyts

Abstract

A chimeric flavivirus infectious cDNA was constructed by exchanging the premembrane (prM) and envelope (E) genes of the yellow fever virus vaccine strain 17D (YF17D) with the corresponding genes of Modoc virus (MOD). This latter virus belongs to the cluster of the "not-known vector" flaviviruses and is, unlike YF17D, neuroinvasive in SCID mice. Replication of in vitro-transcribed RNA from this chimeric flavivirus was shown by [(3)H]uridine labeling and RNA analysis. Expression of the MOD prM and E proteins was monitored by radioimmunoprecipitation and revealed that the MOD proteins were correctly and efficiently produced from the chimeric precursor protein. The MOD E protein was shown to be N-linked glycosylated, whereas prM, as predicted from the genome sequence, did not contain N-linked carbohydrates. In Vero cells, the chimeric virus replicated with a similar efficiency as the parental viruses, although it formed smaller plaques than YF17D and MOD. In SCID mice that had been infected intraperitoneally with the chimeric virus, the viral load increased steadily until death. The MOD/YF virus, like MOD from which it had acquired the prM and E structural proteins, but unlike YF, proved neuroinvasive in SCID mice. Animals develope...Continue Reading

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Jul 19, 2002·The Journal of General Virology·Nathalie CharlierJohan Neyts
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Feb 5, 2003·Journal of Virological Methods·Nathalie CharlierJohan Neyts
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Citations

Mar 9, 2013·Antiviral Research·Tine De BurghgraeveJohan Neyts
May 25, 2012·PloS One·Tine De BurghgraeveJohan Neyts
Mar 24, 2016·PloS One·Dhanasekaran GovindarajanAndrew J Bett
Mar 24, 2017·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Marjan Wouthuyzen-BakkerWouter Fw Bierman
Jun 22, 2017·Viruses·Bradley J Blitvich, Andrew E Firth
Nov 26, 2010·Expert Review of Vaccines·Mohan Babu Appaiahgari, Sudhanshu Vrati
Dec 15, 2018·Reviews in Medical Virology·Tingting ChenWei Zhao
Sep 7, 2021·The Journal of General Virology·Jermilia CharlesBradley J Blitvich

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