Excision of aflatoxin B1-imidazole ring opened guanine adducts from DNA by formamidopyrimidine-DNA glycosylase

Carcinogenesis
C J Chetsanga, G P Frenette

Abstract

This investigation has confirmed the earlier reports that when aflatoxin B1-DNA adducts are stored under physiological conditions some aflatoxin B1-guanine adducts are converted to a secondary product in which fission of the imidazole ring of the adduct guanine has occurred. Incubation of DNA containing aflatoxin B1-guanine adducts for an increasing number of hours under physiological conditions resulted in a progressive increase in the number of adducts in which the imidazole rings of guanines underwent fission. It was shown that the Escherichia coli enzyme, formamidopyrimidine-DNA glycosylase exercises from the 6-day incubated DNA, an amount of imidazole ring opened guanines equivalent to 40% of the aflatoxin B1-guanine adducts present in the DNA. The enzymatic excision of imidazole ring opened aflatoxin B1-guanine adducts is inhibited by Cibacron Blue F3GA a strong inhibitor of formamidopyrimidine-DNA glycosylase. Treatment of aflatoxin B1-DNA with mild alkali (pH 9.6), resulted in a 2-fold increase in the amount of aflatoxin B1-guanines with opened imidazole rings; this was revealed by enzyme assays using this alkaline treated DNA substrate as well as by analysis of acid hydrolysates of the alkaline treated DNA.

Citations

Jan 1, 1989·Molecular & General Genetics : MGG·S Boiteux, O Huisman
May 1, 1993·Mutation Research·J Tchou, A P Grollman
May 25, 2011·Biochemistry·Daniel S HitchcockFrank M Raushel
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C J Chetsanga, C Grigorian
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T R O'Connor, J Laval
Oct 1, 1985·Environmental Health Perspectives·H AutrupC C Harris
Sep 1, 1990·Mutation Research·K Sakumi, M Sekiguchi
Jan 1, 1985·Critical Reviews in Toxicology·Y Ueno
Mar 21, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Stefanie C FleckBeate Köberle
Feb 7, 2006·Cancer Letters·Leanne L Bedard, Thomas E Massey
May 2, 2020·International Journal of Molecular Sciences·Grigory V MechetinDmitry O Zharkov
Apr 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Vladimir VartanianR Stephen Lloyd
Jan 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Martin Klvana, Urban Bren
Mar 22, 2019·DNA Repair·Amanda K McCullough, R Stephen Lloyd
Mar 1, 1992·Mutation Research·R WatersN J Jones
Feb 19, 2002·Chemical Reviews·Sheila S. David, Scott D. Williams

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.