Excision of hydrocarbon-DNA adducts and consequent cell survival in normal and repair defective human cells

Chemico-biological Interactions
B A McCawJ J Roberts

Abstract

Survival curves for normal human cells and xeroderma pigmentosum variant cells (XP4BE) after ultraviolet radiation were indistinguishable. In comparison, cells from xeroderma pigmentosum complementation group A (XP12BE) were very sensitive to ultraviolet radiation. Complementation group C (XP2BE) cells were almost as sensitive as group A cells. These survival phenomena parallel to known unscheduled DNA synthesis responses of these cells to ultraviolet radiation, which, compared with normal cells, are: XP4BE, 100%; XP2BE, 20%; XP12BE, 2%. The relative capacities of these cells to excise 7-bromomethylbenz[a]anthracene-DNA adducts and to survive treatment with the carcinogen were similar to the responses to ultraviolet irradiation, except that the XP2BE cell line both excised and survived this damage far better than anticipated from its response to ultraviolet irradiation. Moreover, whilst in the normal cells and variant cells the ratio of hydrocarbon-adenine adduct to hydrocarbon-guanine adduct remaining in DNA decreased notably with excision, this ratio did not change significantly with excision in the XP2BE cell line. The relationship between greater excision capacity and increased cell survival in the experiments with the chem...Continue Reading

References

Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F E Ahmed, R B Setlow
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D E AmacherM W Lieberman
Jan 1, 1976·Nature·T Lindahl
Dec 1, 1975·Mutation Research·K H KraemerD Bootsma
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D M Kirtikar, D A Goldthwait

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Citations

Jan 1, 1985·Mutation Research·M Protić-SabljićK H Kraemer
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L H ThompsonL E Dillehay
Nov 1, 1980·Photochemistry and Photobiology·F E Ahmed, R B Setlow
Dec 28, 1979·Biochemical and Biophysical Research Communications·A Dipple, M E Hayes
Oct 13, 2006·Culture, Medicine and Psychiatry·Sarah DarghouthCecile Rousseau

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