PMID: 9447231Feb 3, 1998Paper

Excision-repair patch lengths are similar for transcription-coupled repair and global genome repair in UV-irradiated human cells

Mutation Research
K K BowmanP C Hanawalt

Abstract

We have used the buoyant density shift method to measure excision-repair patch lengths in UV-irradiated repair-proficient human cells and in primary fibroblasts belonging to xeroderma pigmentosum complementation group C (XP-C), in which excision repair of UV-induced photoproducts is dependent upon transcription. The patch size was found to be about 30 nucleotides for both cell types. This agrees with the size of the DNA fragments excised in vitro by the dual incisions of the structure-specific nucleases XPG and ERCC1-XPF. We conclude that the XPC protein is not required to target the excision nucleases to sites of DNA cleavage in transcribed strands of expressed genes or to protect the newly incised DNA from further processing by exonucleases.

References

May 19, 1976·Biochimica Et Biophysica Acta·C A Smith, P C Hanawalt
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C A Smith, P C Hanawalt
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J C HuangA Sancar
Sep 1, 1991·Photochemistry and Photobiology·J E CleaverD L Mitchell
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J VenemaL V Mayne
Jan 1, 1987·Journal of Cell Science. Supplement·C A Smith
Jul 19, 1972·Nature: New Biology·E A De Weerd-KasteleinD Bootsma
Jul 20, 1972·Biochimica Et Biophysica Acta·H Edenberg, P Hanawalt
Aug 25, 1983·Nucleic Acids Research·S A LeadonP C Hanawalt
Dec 23, 1994·Science·P C Hanawalt
Feb 10, 1995·The Journal of Biological Chemistry·D MuA Sancar
Jul 29, 1994·Annals of the New York Academy of Sciences·P K Cooper, S A Leadon
Oct 1, 1995·Trends in Biochemical Sciences·A R Lehmann
Apr 5, 1996·The Journal of Biological Chemistry·D MuA Sancar
Aug 6, 1996·Biochemistry·D B ZambleS J Lippard
Jan 1, 1996·Annual Review of Biochemistry·E C Friedberg
Jan 1, 1996·Annual Review of Biochemistry·A Sancar
Jan 1, 1996·Annual Review of Biochemistry·R D Wood

❮ Previous
Next ❯

Citations

Aug 1, 1998·Mutation Research·P C Hanawalt
Apr 24, 1999·Biochimie·S Tornaletti, P C Hanawalt
Feb 16, 2000·Gene·D P Batty, R D Wood
May 9, 2001·Mutation Research·P C Hanawalt
Nov 22, 2008·Nature Reviews. Molecular Cell Biology·Philip C Hanawalt, Graciela Spivak
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·P C HanawaltS Tornaletti
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·S E Tsutakawa, P K Cooper
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·B J HwangG Chu
May 24, 2011·DNA Repair·Alan R Lehmann
Apr 2, 2013·Mechanisms of Ageing and Development·Bruce C McKay, Miguel A Cabrita
Oct 28, 2016·Photochemistry and Photobiology·Michael G Kemp, Jinchuan Hu
Jan 13, 2006·The EMBO Journal·Jean-Philippe Lainé, Jean-Marc Egly

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.