Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks

Scientific Reports
Iurii SemenovAndrei G Pakhomov

Abstract

Intense electric shocks of nanosecond (ns) duration can become a new modality for more efficient but safer defibrillation. We extended strength-duration curves for excitation of cardiomyocytes down to 200 ns, and compared electroporative damage by proportionally more intense shocks of different duration. Enzymatically isolated murine, rabbit, and swine adult ventricular cardiomyocytes (VCM) were loaded with a Ca2+ indicator Fluo-4 or Fluo-5N and subjected to shocks of increasing amplitude until a Ca2+ transient was optically detected. Then, the voltage was increased 5-fold, and the electric cell injury was quantified by the uptake of a membrane permeability marker dye, propidium iodide. We established that: (1) Stimuli down to 200-ns duration can elicit Ca2+ transients, although repeated ns shocks often evoke abnormal responses, (2) Stimulation thresholds expectedly increase as the shock duration decreases, similarly for VCMs from different species, (3) Stimulation threshold energy is minimal for the shortest shocks, (4) VCM orientation with respect to the electric field does not affect the threshold for ns shocks, and (5) The shortest shocks cause the least electroporation injury. These findings support further exploration of ...Continue Reading

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Citations

Dec 26, 2018·Journal of Cardiovascular Electrophysiology·Jan E AzarovAndrei G Pakhomov
Jun 14, 2020·Bioelectromagnetics·Shu XiaoStephen Beebe
Sep 15, 2019·Biomedical Engineering Online·Priscila C AntoneliPedro X de Oliveira
May 15, 2020·International Journal of Molecular Sciences·Wenfei BoAndrei G Pakhomov
Jul 21, 2020·Bioelectricity·Johanna U NeuberChristian W Zemlin
Sep 2, 2019·Biochemical and Biophysical Research Communications·Andrei G PakhomovOlga N Pakhomova
Jul 28, 2020·Bioelectrochemistry·Andrei G Pakhomov, Olga N Pakhomova
Jul 3, 2021·International Journal of Molecular Sciences·Vitalii KimAndrei G Pakhomov
Jul 19, 2021·Bioelectrochemistry·Christian W Zemlin

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Methods Mentioned

BETA
fluorescence imaging

Software Mentioned

MetaMorph Advanced
Clampex
COMSOL Multiphysics
pClamp

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