PMID: 39290Jun 12, 1979

Excitation-contraction coupling in frog sartorius and the role of the surface charge due to the carboxyl group of sialic acid

Pflügers Archiv : European journal of physiology
M Dörrscheidt-Käfer

Abstract

Frog sartorius muscle fibres were incubated with the enzyme neuraminidase which is known to remove surface-bound sialic acids. The sialic acid content of the incubation media was analysed, and the relationship between the threshold of contraction and the altered pH and divalent cation concentration was investigated. The threshold potential of fibres treated with 3.3, 5 or 6.7 units of neuraminidase (at pH 5.5 and 30 degrees C for 2 h) was more positive than that of the control muscle fibres incubated under the same conditions, but without the enzyme. The potential shift is positively correlated with the enzyme concentration and with the amount of sialic acid released. After incubation with 5 units of neuraminidase the potential shift rose to +8.5 mV, depending on [Ca2+]0, [Mg2+]0 and pH. The threshold shift is greatest at low divalent cation concentration (0.5 mM), and not significant at high concentrations of divalent cations (50 mM). In both neuraminidase-treated and control muscles, the effectiveness of Mg2+ is half of that of Ca2+. The dependence of the contraction threshold on pH in the range 5.5--10 is even more pronounced in enzyme-treated than in control muscle fibres. Resting potential, time-course and overshoot of act...Continue Reading

References

Jun 12, 1979·Pflügers Archiv : European journal of physiology·M Dörrscheidt-Käfer
Jan 1, 1983·The Journal of Membrane Biology·M Dörrscheidt-Käfer
Feb 26, 2004·The Journal of General Physiology·Bradley S Launikonis, D George Stephenson
Feb 23, 2010·Molecular & Cellular Proteomics : MCP·T N C RamyaJames C Paulson
Apr 25, 2012·Annals of the New York Academy of Sciences·Ajit Varki, Pascal Gagneux
Jan 1, 1981·The American Journal of Physiology·E W Stephenson

Citations

Jun 10, 1975·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·B HilleB I Shapiro
Jul 19, 1977·Pflügers Archiv : European journal of physiology·M Dörrscheidt-Käfer
Jul 1, 1975·The Journal of General Physiology·B A Mobley, B R Eisenberg
Jan 1, 1970·Physiological Reviews·A M Katz
Jun 20, 1972·Biochimica Et Biophysica Acta·V M Madeira, A P Carvalho
May 1, 1969·Zeitschrift Für Naturforschung. Teil B. Anorganische Chemie, Organische Chemie, Biochemie, Biophysik, Biologie·E Balke, R Drzeniek
Jan 1, 1974·The Journal of Membrane Biology·H H VandenburghS I Zacks
Nov 1, 1966·Experimental Cell Research·S Nordling, E Mayhew
Jan 1, 1970·International Review of Cytology·R J Winzler
Jan 1, 1983·The Journal of Membrane Biology·M Dörrscheidt-Käfer
Jul 1, 1953·The Journal of Physiology·J E DESMEDT

Related Concepts

Calcium
Resting Potentials
Salientia
Neuraminidase
Microtubules
Rana temporaria
Muscle
Inotropism
Sialic Acids
Dose-Response Relationship, Drug

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