PMID: 6974739Sep 1, 1981Paper

Excitation-contraction uncoupling of striated muscle fibres by formamide treatment: evidence of detubulation

Journal of Muscle Research and Cell Motility
V Argiro

Abstract

A reportedly new technique of excitation-contraction uncoupling of frog skeletal muscle fibres was evaluated by low-frequency linear cable analysis and electron microscopy. Results indicate a similarity between the electrophysiological and ultrastructural effects of the formamide procedure and those obtained with conventional glycerol shock. Fibres in isolated frog sartorius muscles immersed in a frog Ringer's-formamide solution showed a rapid decline in twitch tension and a decrease in specific membrane capacitance, and disruption of the morphology of the transverse tubule system. These results are consistent with physical and electrical isolation of the transverse tubular network from the surface membrane of the muscle fibre ("detubulation"). However, reduction of twitch tension is marked in the formamide solution. Complete paralysis is only achieved following the return to normal Ringer's. Probably due to the greater membrane permeability of formamide, the technique requires less time and may be more reliable than the standard glycerol shock procedure.

References

Sep 1, 1978·The Journal of Cell Biology·J del Castillo, G Escalona de Motta
Jan 1, 1973·The Journal of Membrane Biology·C Franzini-ArmstrongP Horowicz
Feb 1, 1972·The Journal of Physiology·A L Hodgkin, S Nakajima
Sep 1, 1968·Life Sciences·F CórdobaJ del Castillo
Mar 1, 1969·The Journal of General Physiology·P W Gage, R S Eisenberg
Dec 3, 1946·Proceedings of the Royal Society of Medicine·A L HODGKIN, W A H RUSHTON

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Citations

Oct 1, 1996·The American Journal of Physiology·R W Putnam
Oct 1, 1995·Journal of Muscle Research and Cell Motility·G KoutsisC L Huang

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