Excited-state lifetime studies of the three tryptophan residues in the N-lobe of human serum transferrin.

Protein Science : a Publication of the Protein Society
Nicholas G JamesD M Jameson

Abstract

The energy transfer from the three Trp residues at positions 8, 128, and 264 within the human serum transferrin (hTF) N-lobe to the ligand to metal charge transfer band has been investigated by monitoring changes in Trp fluorescence emission and lifetimes. The fluorescence emission from hTF N-lobe is dominated by Trp264, as revealed by an 82% decrease in the quantum yield when this Trp residue is absent. Fluorescence lifetimes were determined by multifrequency phase fluorometry of mutants containing one or two Trp residues. Decays of these samples are best described by two or three discrete lifetimes or by a unimodal Lorentzian distribution. The discrete lifetimes and the center of the lifetime distribution for samples containing Trp128 and Trp264 are affected by iron. The distribution width narrows on iron removal and is consistent with a decrease in dynamic mobility of the dominant fluorophore, Trp264. Both the quantum yield and the lifetimes are lower when iron is present, however, not proportionally. The greater effect of iron on quantum yields is indicative of nonexcited state quenching, i.e., static quenching. The results of these experiments provide quantitative data strongly suggesting that Förster resonance energy tran...Continue Reading

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Citations

Nov 17, 2010·Analytical Biochemistry·Martin SteflDavid M Jameson
Aug 23, 2011·Biochimica Et Biophysica Acta·Alex D SheftelPrem Ponka
Apr 3, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Martín E NogueraJavier Santos
Mar 8, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Pavel KasplerLothar Lilge
Jun 27, 2017·Chemistry, an Asian Journal·Isabel CorreiaJoão Costa Pessoa
Mar 8, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Nicoleta SanduAurel I Popescu
Jun 9, 2021·Journal of Biomolecular Structure & Dynamics·Nicoleta SanduClaudia G Chilom
Nov 17, 2010·Analytical Biochemistry·Nicholas G JamesDavid M Jameson
Jan 13, 2012·Chemistry : a European Journal·Kevin GuoMikhail Y Berezin
Jun 20, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Lydie BrissonA Jalila Simaan
Sep 27, 2012·Journal of Inorganic Biochemistry·Olga MazurykMałgorzata Brindell

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