Excited state proton transfer and solvent relaxation of a 3-hydroxyflavone probe in lipid bilayers

The Journal of Physical Chemistry. B
Ranjan DasYves Mély

Abstract

The photophysics of a ratiometric fluorescent probe, N-[[4'- N, N-diethylamino-3-hydroxy-6-flavonyl]methyl]- N-methyl- N-(3-sulfopropyl)-1-dodecanaminium, inner salt (F2N12S), incorporated into phospholipid unilamellar vesicles is presented. The reconstructed time-resolved emission spectra (TRES) unravels a unique feature in the photophysics of this probe. TRES exhibit signatures of both an excited-state intramolecular proton transfer (ESIPT) and a dynamic Stokes shift associated with solvent relaxation in the lipid bilayer. The ESIPT is fast, being characterized by a risetime of approximately 30-40 ps that provides an equilibrium to be established between the excited normal (N*) and the ESIPT tautomer (T*) on a time scale of 100 ps. On the other hand, the solvent relaxation displays a bimodal decay kinetics with an average relaxation time of approximately 1 ns. The observed slow solvent relaxation dynamics likely embodies a response of nonspecific dipolar solvation coupled with formation of probe-water H-bonds as well as the relocation of the fluorophore in the lipid bilayer. Taking into account that ESIPT and solvent relaxation are governed by different physicochemical properties of the probe microenvironment, the present stu...Continue Reading

References

Jun 1, 1990·Biophysical Journal·T ParasassiE Gratton
Jun 13, 1986·Biochimica Et Biophysica Acta·L D MayerP R Cullis
Apr 7, 1998·Biochimica Et Biophysica Acta·O P BondarE S Rowe
Jul 2, 1998·Biophysical Journal·L A BagatolliG D Fidelio
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·D Marsh
Nov 26, 2002·Chemistry & Biology·Andrey S KlymchenkoAlexander P Demchenko
Jan 28, 2003·Biophysical Journal·Rosa BartucciLuigi Sportelli
Apr 28, 2004·Biophysical Journal·Andrey S KlymchenkoYves Mély
Oct 9, 2004·Biochimica Et Biophysica Acta·Andrey S KlymchenkoGuy Duportail
Sep 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Lennart Nilsson, Bertil Halle
Oct 4, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Piotr JurkiewiczMartin Hof
Oct 20, 2006·Nature Chemical Biology·Joshua Zimmerberg, Klaus Gawrisch
Jan 30, 2007·Journal of the American Chemical Society·Vasyl V ShynkarYves Mely

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Citations

Oct 29, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Ranjan DasYves Mély
Nov 23, 2013·The Journal of Physical Chemistry. B·Štěpán TimrPavel Jungwirth
Mar 27, 2012·Annual Review of Biochemistry·Liguo Wang
Dec 15, 2015·The Journal of Physical Chemistry. a·Deborin GhoshDebabrata Mandal
May 8, 2014·Chemistry and Physics of Lipids·Roopali SaxenaAmitabha Chattopadhyay
May 6, 2009·Biophysical Journal·Alexander P DemchenkoAndrey S Klymchenko
Oct 30, 2016·The Journal of Physical Chemistry. B·Saikat BiswasPramit Kumar Chowdhury
Mar 29, 2014·Physical Chemistry Chemical Physics : PCCP·Deborin GhoshDebabrata Mandal
Feb 17, 2017·Physical Chemistry Chemical Physics : PCCP·Aurelie PerveauxDavid Lauvergnat
Nov 26, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Monalisa Mohapatra, Ashok Kumar Mishra
Apr 3, 2020·Acta Neuropathologica·Joseph BenetatosJürgen Götz
Dec 15, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Nilanjan DeySantanu Bhattacharya
Feb 27, 2010·The Journal of Physical Chemistry. a·Hui-Hsu Gavin TsaiChun-Jui Tan
Nov 27, 2014·The Journal of Physical Chemistry. B·Ymène HouariAdèle D Laurent

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