Excretion in the house cricket Acheta domesticus: Effects of diuretics on the structure of the mid-tubule

Journal of Morphology
J H Spring, Bruce E Felgenhauer

Abstract

Most structural studies of insect Malpighian tubules focus on freshly dissected tissue, which is in fact stimulated by diuretic factors. In this study, we examine tubules from the house cricket Acheta domesticus in four discrete secretory states: control (freshly dissected); unstimulated (held in vitro for 90 min prior to fixation); corpus cardiacum-stimulated (held in vitro for 60 min, then stimulated with corpus cardiacum homogenates for 30 min prior to fixation); and cAMP-stimulated (held in vitro for 60 min, then stimulated with dibutyryl cAMP for 30 min prior to fixation). In unstimulated tubules, we see a reduction in vacuolization and a near-complete collapse of the basolateral infolds. Stimulated tubules show several major structural shifts: mitochondria are darkly stained with well-defined cristae, there is extensive vacuolization of the tissue and expansion of the basolateral spaces, and the CaPO4 spherites appear to be ejected into the lumen. cAMP-stimulated tubules showed the most pronounced structural changes, including the presence of a newly reported ultrastructural feature for A. domesticus Malpighian tubules, referred to here as paracrystalline arrays, which appear as stacks of membrane localized in the perinuc...Continue Reading

References

Jan 1, 1979·Journal of Supramolecular Structure·T J Bradley, P Satir
May 28, 1986·Biochimica Et Biophysica Acta·H WieczorekW R Harvey
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S GluckQ Al-Awqati
Jan 1, 1995·Archives of Insect Biochemistry and Physiology·J H Spring, I Kim
Aug 26, 1988·Science·J H SpringS R Hazelton

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Citations

May 18, 2000·Microscopy Research and Technique·V R TownsendJ H Spring
May 2, 2000·Archives of Insect Biochemistry and Physiology·K A DubeM J O'Donnell
Nov 22, 2017·Archives of Insect Biochemistry and Physiology·Austin Browne, Michael J O'Donnell
Mar 22, 2021·The Science of the Total Environment·Mark TibbettJeanette Whitaker
Feb 3, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jeffrey H SpringJeffrey L Brodsky

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