PMID: 6990888Jan 1, 1980Paper

Excretion of a protease by Serratia marcescens

Archives of Microbiology
V Braun, G Schmitz

Abstract

Excretion of an extracellular protease of Serratia marcescens ATCC 25419 occurred during logarithmic growth and was highest (per cell) when cultures reached the stationary growth phase. Production of the extracellular protease was induced by leucine or casein in minimal medium or by growth in tryptone-yeast medium. In the late stationary phase an intracellular protease activity accumulated which was also observed in mutants with very low extracellular protease activity. The excreted protease was the dominant protein in the growth medium. The protease was purified to homogeneity by column chromatography on Bio-Gel P-100 and on DEAE-cellulose. Quantitative amino acid analysis revealed the absence of sulfur-containing amino acids. The enzyme consists of one polypeptide chain. A molecular weiht of 51,000 and 55,000 was estimated using polyacrylamide gel electrophoresis and chromatography on Bio-Gel P-100 respectively. The enzyme cleaved only N-alpha-benzoyl-DL-lysine-and-arginine-nitroanilides but not the corresponding leucine or tyrosine derivatives nor a set of di- and tripeptides.

References

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Citations

May 1, 1985·Archives of Microbiology·V BraunC Tautz
Jul 1, 1990·Molecular & General Genetics : MGG·S C Braunagel, M J Benedik
Aug 2, 2007·Journal of Industrial Microbiology & Biotechnology·Hyun-Soo KimKenneth N Timmis
Sep 10, 2013·Canadian Journal of Microbiology·Lauren M Petersen, Louis S Tisa
Mar 1, 1987·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·W H Traub, D Bauer
Jan 1, 2006·Molecular Plant Pathology·Sarah J CoulthurstGeorge P C Salmond
Jul 31, 2013·Molecular Microbiology·Lynne S CairnsNicola R Stanley-Wall
Dec 1, 1982·The Journal of Applied Bacteriology·R C McKellar
Feb 18, 2006·International Journal of Medical Microbiology : IJMM·Jun-Rong Wei, Hsin-Chih Lai
Oct 24, 2013·Microbiology·Victoria L MarlowNicola R Stanley-Wall
Jun 1, 1990·FEMS Microbiology Letters·J PalomarM Viñas
Feb 1, 1986·The Journal of Dairy Research·D J Fairbairn, B A Law
Aug 5, 2008·Bioorganicheskaia khimiia·N V MozhinaS V Kostrov
Aug 3, 2013·Journal of Food Protection·Dong Hwan LeeSunggi Heu
Jan 12, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Dongxia WuDongqing Xu
Apr 5, 2001·Journal of Biomedical Science·S AngS Swift
Feb 1, 1992·FEMS Microbiology Immunology·B L WetherallA M Johnson
Aug 26, 1998·FEMS Microbiology Letters·M J Benedik, U Strych
Jul 1, 1981·Journal of Bacteriology·A P Pugsley, J P Rosenbusch
Apr 1, 1981·Journal of Bacteriology·K SchallerV Braun
Nov 1, 1992·Journal of Bacteriology·R A Salomón, R N Farías
Dec 1, 1990·Journal of Bacteriology·N Hugouvieux-Cotte-PattatS Harayama

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