Excretion of acetylated and free polyamines by polyamine depleted Chinese hamster ovary cells

The International Journal of Biochemistry
T Hyvönen

Abstract

1. Cultured Chinese hamster ovary cells (CHO) and their ornithine decarboxylase deficient mutant cells (C55.7) were found to excrete small amounts of N8-acetylspermidine and free polyamines, putrescine and spermidine into the culture medium. 2. The concentration of N8-acetylspermidine in the control cells was 2-3% of that of spermidine. In the medium, however, the amount of N8-acetylspermidine was about 2-fold that of spermidine and 2- to 3-fold higher than the intracellular amount. N1-acetylspermidine or acetylated spermine were never detected in the cells or in the media. 3. Confluent CHO cells treated with 2 mM difluoromethylornithine stopped the excretion when the intracellular spermidine concentration had decreased to 20% of control while there was no decrease in spermine concentration. At low cell density, neither polyamine depleted CHO cells nor the C55.7 cells excreted any polyamines into the culture media.

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Citations

Sep 1, 1992·The International Journal of Biochemistry·B FulgosiM A Grillo
Aug 1, 1996·The International Journal of Biochemistry & Cell Biology·N SeilerJ P Moulinoux
Nov 11, 2009·Rapid Communications in Mass Spectrometry : RCM·William P K ChongYing Swan Ho
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