Excretion of Morroniside in Rat Urine After Single Oral and Intravenous Administration

Journal of Chromatographic Science
Shan XiongZhenqing Zhang

Abstract

This study was designed to develop a sensitive, simple and rapid method for the quantitation of morroniside in rat urine using high-performance liquid chromatography-tandem mass spectrometry (LC-MS-MS) and to investigate the excretion of morroniside in rat urine. The mobile phase consisted of water-acetonitrile (gradient elution) at a flow rate of 0.4 mL/min. Detection was performed using positive-ion electrospray ionization in multiple reaction monitoring (MRM) modes. And the detection of morroniside in rat urine by the LC-MS-MS was accurate and precise from 1.0 to 2,500 ng/mL (a correlation coefficient of 0.9953). The recoveries and matrix effects were all in line with the biological sample measurement requirements. The intraday accuracy was 88.68-105.78% with precision of 6.50-11.19% and the interday accuracy was 95.77-102.43% with precision of 7.08-10.40%. Excretion data of morroniside in rat urine indicated that 21.43‰ (i.g.) and 100.35% (i.v.) of the dose administered was excreted as unconverted form, respectively. And the maximal excretion rate was 27.57 and 482.42 μg/h after oral and intravenous administration, respectively. These results indicated that the developed method has satisfactory sensitivity, accuracy and pre...Continue Reading

References

Oct 6, 2009·Biological & Pharmaceutical Bulletin·Chan Hum ParkTakako Yokozawa
Jul 20, 2010·Brain Research Bulletin·Wen WangLin Li
Oct 23, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Manyu LiXijun Wang
Jan 16, 2014·Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·Shan XiongZhen-Qing Zhang

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Citations

Jul 2, 2019·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Shan XiongZhenqing Zhang

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