Exercise-induced changes in beta-adrenergic-receptor mRNA level measured by competitive RT-PCR

Journal of Applied Physiology
N FujiiH Miyazaki

Abstract

Competitive reverse transcription-polymerase chain reaction (RT-PCR) analysis was used to clarify whether dynamic exercise-induced increases in beta-adrenergic-receptor (beta-AR) number in human lymphocytes are accompanied by increases in the beta-AR mRNA level. Sixteen healthy subjects performed cycle ergometry until exhaustion. Before and immediately after exercise, peripheral blood was drawn from a forearm vein for preparation of lymphocytes. Both the beta-AR mRNA level and the beta-AR number were significantly increased by exercise. The changes in beta-AR mRNA level and beta-AR number were significantly correlated (r = 0.63, P < 0.01). This finding suggests that a rapid increase in beta-AR mRNA level might be an early adaptive response of the sympathetic nervous system to dynamic exercise. In vitro incubation of lymphocytes with epinephrine had no effect on beta-AR mRNA levels, nor did adenosine 3',5'-cyclic monophosphate, protein kinase C, or intracellular Ca2+ increase the beta-AR mRNA level in vitro. Therefore, it appears that other mechanisms underlie the exercise-induced elevation of beta-AR mRNA levels in human lymphocytes.

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Citations

Sep 2, 2003·Molecular Reproduction and Development·Béla Endre ReschGeorge Falkay
Feb 13, 2019·Cardiovascular Drugs and Therapy·Sophie N SaxtonAnthony M Heagerty
Jun 5, 1998·The American Journal of Physiology·N FujiiH Miyazaki

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