Exercise-Induced Changes in Caveolin-1, Depletion of Mitochondrial Cholesterol, and the Inhibition of Mitochondrial Swelling in Rat Skeletal Muscle but Not in the Liver

Oxidative Medicine and Cellular Longevity
Damian Jozef FlisWieslaw Ziolkowski

Abstract

The reduction in cholesterol in mitochondria, observed after exercise, is related to the inhibition of mitochondrial swelling. Caveolin-1 (Cav-1) plays an essential role in the regulation of cellular cholesterol metabolism and is required by various signalling pathways. Therefore, the aim of this study was to investigate the effect of prolonged swimming on the mitochondrial Cav-1 concentration; additionally, we identified the results of these changes as they relate to the induction of changes in the mitochondrial swelling and cholesterol in rat skeletal muscle and liver. Male Wistar rats were divided into a sedentary control group and an exercise group. The exercised rats swam for 3 hours and were burdened with an additional 3% of their body weight. After the cessation of exercise, their quadriceps femoris muscles and livers were immediately removed for experimentation. The exercise protocol caused an increase in the Cav-1 concentration in crude muscle mitochondria; this was related to a reduction in the cholesterol level and an inhibition of mitochondrial swelling. There were no changes in rat livers, with the exception of increased markers of oxidative stress in mitochondria. These data indicate the possible role of Cav-1 in ...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·R L LevineE R Stadtman
Sep 26, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A ColellJ C Fernández-Checa
May 6, 2003·Analytical Biochemistry·Elliott D CrouserDouglas R Pfeiffer
Mar 9, 2004·Genome Biology·Terence M Williams, Michael P Lisanti
Sep 5, 2006·Cell Metabolism·Montserrat MaríCarmen García-Ruiz
Feb 24, 2007·Nature Reviews. Molecular Cell Biology·Robert G Parton, Kai Simons
Jan 16, 2008·Sub-cellular Biochemistry·P Bernardi, A Rasola
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Elina Ikonen
Dec 2, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Morvarid Shir MohammadiKhalid A Tazi
Feb 24, 2009·Journal of Hepatology·Francisco CaballeroCarmen García-Ruiz
May 28, 2011·Experimental Gerontology·M S Dhivya VadhanaRosita Gabbianelli
Aug 26, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kyle S McCommisChristopher P Baines
Apr 5, 2012·Laboratory animal research·Sookyoung ParkYonggeun Hong
Jun 5, 2013·Experimental Physiology·Wieslaw ZiolkowskiRosita Gabbianelli
May 13, 2014·The American Journal of Pathology·Elisabet Barbero-CampsAnna Colell
Jul 6, 2014·Biochemical and Biophysical Research Communications·Jung-Woo Kang, Sun-Mee Lee
Nov 27, 2014·Journal of Bioenergetics and Biomembranes·Laura A MartinBarbara Karten

❮ Previous
Next ❯

Citations

Jul 12, 2018·Menopause : the Journal of the North American Menopause Society·Wissuta SuthamSiriporn C Chattipakorn
Nov 30, 2018·International Journal of Molecular Sciences·Konrad KleszczyńskiAndrzej T Slominski

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA

Software Mentioned

Statistica

Related Concepts

Related Feeds

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.