Exercise training reverses insulin resistance in muscle by enhanced recruitment of GLUT-4 to the cell surface

The American Journal of Physiology
G J EtgenJohn L Ivy

Abstract

The effects of exercise training on cell surface GLUT-4 in skeletal muscle of the obese (fa/fa) Zucker rat were investigated using the impermeant glucose transporter photoaffinity reagent 2-N-4-(1-azi-2,2,2-trifluoroethyl)-benzoyl-1,3-bis- (D-mannos-4-yloxy)-2-propylamine (ATB-BMPA). In the absence of insulin, 3-O-methyl-D-glucose transport activity was no different in either fast-twitch (epitrochlearis) or slow-twitch (soleus) muscles of trained and sedentary obese rats. Likewise, basal ATB-BMPA-labeled GLUT-4 was not altered in these muscles with training. In contrast, the trained group exhibited significantly greater insulin-stimulated (2 mU/ml) glucose transport activity in epitrochlearis muscles than the sedentary group (0.53 +/- 0.03 vs. 0.18 +/- 0.03 mumol.g-1 x 10 min-1 for trained and sedentary, respectively), which was paralleled by a significant enhancement of insulin-stimulated cell surface GLUT-4 (5.33 +/- 0.20 vs. 1.57 +/- 0.14 disintegrations.min-1.mg-1 for trained and sedentary, respectively). Exercise training, however, did not alter insulin-stimulated glucose transport activity or cell surface GLUT-4 in soleus muscles. Finally, exercise training did not alter the ability of muscle contraction to elevate glucos...Continue Reading

Citations

Jun 14, 2000·Journal of Applied Physiology·T H ReynoldsS W Cushman
Aug 18, 2001·Journal of Applied Physiology·W W Winder
Apr 8, 2004·American Journal of Physiology. Endocrinology and Metabolism·Vitoon SaengsirisuwanErik J Henriksen
Jun 21, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Mohamad SebaiRobert L Hester
Apr 21, 2018·Journal of Exercise Nutrition & Biochemistry·In Ho LeeTae-Dong Kwon
Jul 23, 2002·Journal of Applied Physiology·Erik J Henriksen
Mar 22, 2007·American Journal of Physiology. Endocrinology and Metabolism·Angela C SmithDavid J Dyck
Jul 12, 2007·American Journal of Physiology. Endocrinology and Metabolism·Ben B YaspelkisJohn A Hawley
Aug 5, 2000·Journal of Applied Physiology·K R FoianiniE J Henriksen
Jun 16, 2001·Journal of Applied Physiology·V SaengsirisuwanE J Henriksen
Jan 18, 2002·Journal of Applied Physiology·Christine Y ChristJohn L Ivy
Jun 14, 2000·Journal of Applied Physiology·W W WinderJ O Holloszy
Dec 10, 2009·American Journal of Physiology. Endocrinology and Metabolism·Michael A KirályMichael C Riddell
Mar 3, 2005·American Journal of Physiology. Endocrinology and Metabolism·Jérôme Ruzzin, Jørgen Jensen
Jun 9, 2019·The Journal of Biological Chemistry·Amira KlipDavid E James

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