EXIRAD-3D: Fast automated three-dimensional autoradiography.

Nuclear Medicine and Biology
Minh Phuong NguyenFreek J Beekman

Abstract

Autoradiography is an established technique for high-resolution imaging of radiolabelled molecules in biological tissue slices. Unfortunately, creating a 3D image from a set of these 2D images is extremely time-consuming and error-prone. MicroSPECT systems provide such 3D images but have a low resolution. Here we present EXIRAD-3D, a fast automated method as an alternative for 3D autoradiography from coupes based on ultra-high resolution microSPECT technology. EXIRAD-3D uses a very small bore focusing multi-pinhole collimator mounted in a SPECT system with stationary detectors (U-SPECT/CT, MILabs B.V. The Netherlands) using a sample holder with integrated tissue cooling to avoid activity leaking or tissue deformation during the scan. The system performance was experimentally evaluated using various phantoms and tissue samples of animals in vivo injected with technetium-99m and iodine-123. The reconstructed spatial resolution obtained with a Derenzo hot rod phantom was 120 μm (or 1.7 nl). The voxel values of a syringe phantom image appear to be uniform and scale linearly with activity. Uptake in tiny details of the mouse knee joint, thyroid, and kidney could be clearly visualized. EXIRAD-3D opens up the possibility for fast and ...Continue Reading

Citations

Nov 25, 2020·Physics in Medicine and Biology·Minh Phuong NguyenFreek J Beekman
May 29, 2021·Physics in Medicine and Biology·Minh Phuong NguyenFreek J Beekman
Sep 8, 2021·Frontiers in Public Health·Tatjana PauneskuGayle E Woloschak

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