Abstract
Aqueous extracts from rabbit organs were prepared by homogenization and centrifugation at 105,000 g. After precipitation with ammonium sulphate, the 0-50 fraction was separated by ultrafiltration through Amicon XM 100 and XM 300 membranes yielding two filtrate fractions (U1 and U2) and one retentate fraction (U3). Only U1 and U3 inhibited thymidine incorporation into DNA. After a single injection of U1 from rabbit small intestine, the uptake of tritiated thymidine was decreased in mouse jejunal and colonic DNA. This effect, totally reversible after 7 hr, was found in neither the kidney nor the testis. The U1 fractions of colon and non-digestive organs (kidney, testis) were found not to exert a significant inhibition on thymidine incorporation into intestinal DNA in vivo. The U3 fraction from rabbit small intestine also decreased the uptake of tritiated thymidine in mouse jejunal and colonic DNA in vivo. However, this inhibition was irreversible and not tissue-specific. Slowing of cell migration was also noticed in the jejunum of mice injected with U1 or U3, as ascertained radioautographically by determining the position of the leading edge of the labelled cells in U1- or U3-injected mice compared with controls. A decrease of mi...Continue Reading
References
Mar 1, 1975·Cell and Tissue Kinetics·G Brugal, J Pelmont
Feb 1, 1975·Life Sciences·W S Bullough
Nov 1, 1978·Cell and Tissue Kinetics·P Sassier, M Bergeron
Jul 18, 1975·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·H S Al-DewachiA J Watson
May 1, 1977·Cell and Tissue Kinetics·P Sassier, M Bergeron
Jun 1, 1976·Canadian Journal of Physiology and Pharmacology·P Sassier, M Bergeron
Jan 1, 1975·Gut·P ChanT A Connor
May 1, 1971·The American Journal of Anatomy·W W Chang, C P Leblond
Sep 1, 1972·The British Journal of Dermatology·W S Bullough
May 15, 1973·FEBS Letters·J C Houck, H Hennings
Dec 1, 1974·The American Journal of Anatomy·H Cheng, C P Leblond
Dec 1, 1974·The American Journal of Anatomy·H Cheng, C P Leblond
Oct 1, 1973·Physiological Reviews·M Lipkin
May 1, 1973·Cell and Tissue Kinetics·P J Tutton
Sep 1, 1965·Experimental Cell Research·A B CairnieG G Steel
Jun 1, 1959·Experimental Cell Research·H QUASTLER, F G SHERMAN
Oct 1, 1960·The American Journal of Digestive Diseases·B MESSIER
Citations
Jan 1, 1987·Gut·P RampalC Zunino
Jan 1, 1982·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·A I Al-Nafussi, N A Wright
Jan 1, 1988·Journal of Microencapsulation·M V BogoevaB A Botev
Jan 1, 1994·Growth Factors·C S Potten, C A Chadwick
Oct 7, 1982·Journal of Theoretical Biology·N F BrittonJ D Murray
May 1, 1986·Cell and Tissue Kinetics·E C Jazwinska
Dec 1, 1980·Cell Biophysics·C Nicolini