Existence of Diverse Modifications in Small-RNA Species Composed of 16-28 Nucleotides.

Chemistry : a European Journal
Meng-Dan LanYu-Qi Feng

Abstract

RNA contains diverse modifications that exert an important influence in a variety of cellular processes. So far, more than 150 modifications have been identified in various RNA species, mainly in ribosomal RNA (rRNA), transfer RNA (tRNA), and messenger RNA (mRNA). In contrast to rRNA, tRNA, and mRNA, the known modifications in small RNA species have been primarily limited to 2'-O-ribose methylation in plants and inosine in mammals. The methylation of small RNAs in mammals is still unclear. Current methods widely used in the characterization of small RNAs are mainly based on the strategy of nucleic acid hybridization and sequencing, which cannot characterize modifications in small RNAs. Herein, we have systematically investigated modifications in small RNAs composed of 16-28 nucleotides (nt) by establishing an effective isolation and neutral enzymatic digestion of small RNAs in combination with liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). This method allowed us to simultaneously detect 57 different types of nucleoside modification. By using this approach, we revealed 24 modifications in small RNAs comprising 16-28 nt from human cells. In addition, we found that the obesity-associated pro...Continue Reading

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Citations

Jun 9, 2018·Non-coding RNA·Laura J Leighton, Timothy W Bredy
Oct 13, 2019·Cardiovascular Research·Anne Yaël Nossent
Dec 29, 2019·Cells·Reginald V C T van der KwastA Yaël Nossent
Jan 8, 2020·Chemical Science·Qing-Yun ChengYu-Qi Feng
Aug 23, 2019·Chemical Research in Toxicology·Bei ChenYu-Qi Feng
Sep 14, 2018·Analytical Chemistry·Bei ChenYu-Qi Feng
Mar 29, 2019·Chemical Research in Toxicology·Jun XiongYu-Qi Feng
Jun 13, 2019·ACS Chemical Biology·Cheng-Jie MaYu-Qi Feng

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