PMID: 8605228Feb 9, 1996Paper

Existence of phosphorylated and dephosphorylated forms of cytosolic thymidine kinase (TK1)

Biochimica Et Biophysica Acta
Q HeB Tribukait

Abstract

In this study we examine whether different TK1 variants of pI 6.9 and 8.3 found by isoelectric focusing gel electrophoresis (IFE) reflect just a phenotype difference due to phosphorylation modifications or have a real phenotypic background. The phosphorylation degree of purified TK1 variants was analyzed by determining the changes in the pI values after treatment with alkaline phosphatase, using IFE. The genetic origin of the two TK1 variants was studied by determining their mol wt. by means of SDS-gelelectrophoresis. Furthermore, the subcellular distribution of the two TK1 variants was also studied. Alkaline phosphatase treatment changed the pI value of purified TK1 from 6.9 to 8.3. No change in the pI value was found when purified TK1 corresponding to pI 8.3 was treated in the same way. Similar results were obtained when treated a cytosolic fraction with alkaline phosphatase. Antibody raised against the C-terminal part of human TK1 only recognized the dephosphorylated TK1 variant corresponding to pI 8.3. There was no difference in the molecular weight between the two TK1 variants. Thus, we concluded that the TK1 variants corresponding to pI 6.9 and 8.3 are of the same genetic origin, but consist of phosphorylated and dephosph...Continue Reading

References

Jun 15, 1979·Molecular and Cellular Biochemistry·G TesoriereG Taibi
Dec 1, 1990·Scandinavian Journal of Gastroenterology·I S MenziesB Creamer
Sep 1, 1988·Analytical Biochemistry·D F HochstrasserC R Merril
Sep 2, 2011·Chemistry : a European Journal·Henri A van KalkerenFloris L van Delft

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Citations

May 19, 2010·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Zhishan LiSven Skog
Jun 20, 1998·The Journal of Biological Chemistry·Z F ChangL M Chi

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