PMID: 2508751Oct 9, 1989Paper

Exocytotic enzyme release from rabbit polymorphonuclear leukocytes after treatment with fluoride and calcium

Biochimica Et Biophysica Acta
J G Elferink, M Deierkauf

Abstract

Treatment of rabbit polymorphonuclear leukocytes (PMNs) with 20 mM sodium fluoride for 10 min, followed by removal of fluoride and addition of Ca2+ results in extensive exocytosis. This is apparent from a strong lysozyme release, together with a slight LDH release. During fluoride-activated Ca2+-dependent exocytosis an increase of indo fluorescence and a strong association of 45Ca with the cells occurs. Different inhibitors inhibit both 45Ca association and lysozyme release. Pretreatment of PMNs with pertussis toxin, or the presence of AI3+ in the medium has little effect on fluoride-activated Ca2+-dependent exocytosis. During pretreatment with fluoride, the ATP level strongly decreases. Exocytosis nevertheless occurs upon addition of Ca2+, indicating that a normal ATP level is not required for exocytosis. The glycogen content of the cell strongly decreases during exposure to Ca2+ after pretreatment with fluoride, but not during pretreatment with fluoride. Breakdown of glycogen and accumulation of 3-phosphoglycerate suggest that glycolysis is blocked at the enolase step, but proceeds as far as that step.

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Citations

Aug 1, 1992·International Journal of Immunopharmacology·B A HartP H Nibbering
Jul 1, 1993·International Journal of Immunopharmacology·J G Elferink, B M de Koster
Jul 28, 1993·Biochimica Et Biophysica Acta·G J BoonenJ G Elferink
Jan 17, 1993·Biochimica Et Biophysica Acta·G J BoonenJ G Elferink

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