PMID: 7018908May 15, 1981Paper

Exoenzymatic activity of transglycosylase isolated from Escherichia coli

European Journal of Biochemistry
E H BeacheyU Schwarz

Abstract

The possibility that murein transglycosylase of Escherichia coli may function as an exoenzyme to cleave the murein sacculus in a systematic fashion was investigated. Two molecular species of this hydrolytic enzyme have been isolated and characterized: one is associated with the soluble fraction and the other with the envelope fraction of ruptured E. coli cells. The soluble enzyme was employed to digest murein sacculi that had been uniformly labeled with [3H]diaminopimelic acid. The analysis of the reaction product indicated that the enzyme did not cleave the glycan chains randomly. To determine whether transglycosylase released muropeptide first from the N-acetylglucosaminyl or the 1,6-anhydromuramyl ends of the glycan chains, the [3H]diaminopimelate-labeled sacculi were further radiolabeled at their N-acetylglucosaminyl ends with [14C]galactose by a galactosyl transferase reaction. The transglycosylase released galactose-labeled X + X' muropeptides early during the course of digestion, suggesting exoenzymatic cleavage of the glycan chains preferentially from the N-acetylglucosaminyl ends. (X = N-acetylglucosaminyl-1,6-anhydro-N-acetylmuramyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid; X' = X-D-alanine.) The kinetics of...Continue Reading

References

Dec 1, 1975·Journal of Bacteriology·J V HöltjeU Schwarz
Nov 1, 1978·Journal of Bacteriology·R W VerwerU Schwarz
Sep 1, 1979·Journal of Bacteriology·W Keck, U Schwarz
Feb 25, 1972·Nature·R HartmannU Schwarz
May 10, 1974·Annals of the New York Academy of Sciences·J V Höltje, U Schwarz
May 14, 1969·Journal of Molecular Biology·U SchwarzH Frank
May 1, 1971·Journal of Bacteriology·U Schwarz, W Leutgeb
Oct 1, 1980·Journal of Bacteriology·H MettU Schwarz

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Citations

Oct 1, 1995·Archives of Microbiology·J V Höltje
Feb 1, 1991·Research in Microbiology·T RomeisJ V Höltje
Feb 1, 1991·Research in Microbiology·M CaparrósM A de Pedro
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·J V Höltje
Mar 22, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Yolande A ChanJoseph P Dillard
Jul 1, 1993·Molecular Microbiology·J R CastónJ L Carrascosa
May 2, 1985·European Journal of Biochemistry·W KeckU Schwarz
Nov 7, 2006·Journal of Bacteriology·Catherine ChaputIvo G Boneca
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jean van Heijenoort
Jan 16, 2008·FEMS Microbiology Reviews·Waldemar VollmerMiguel A de Pedro
Jun 24, 2017·Critical Reviews in Biochemistry and Molecular Biology·David A DikShahriar Mobashery
Jan 23, 2020·Physical Review. E·Garima Rani, Issan Patri
Aug 1, 1993·FEMS Microbiology Letters·T RomeisJ V Höltje
Jun 27, 1998·Journal of Bacteriology·A R KraftJ V Höltje
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·J V Höltje
Mar 1, 1997·Journal of Bacteriology·W VollmerV Braun
Sep 1, 1995·Journal of Bacteriology·J C QuintelaM A de Pedro
Sep 1, 1991·Journal of Bacteriology·B Walderich, J V Höltje
Jun 1, 1991·Journal of Bacteriology·U Kohlrausch, J V Höltje

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Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

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