Exogastrula formation in Xenopus laevis embryos depleted with maternal XmN-cadherin mRNA by antisense S-oligo DNA

Biochemical and Biophysical Research Communications
T HojyoKoichiro Shiokawa

Abstract

Xenopus XmN-cadherin gene appears to have dual functions, since its mRNA is maternally provided in unfertilized eggs, once disappears almost completely during gastrula stage, then accumulates again specifically in neural tissues in later stage embryos. In the present experiment, we first followed the change in XmN-cadherin mRNA level during oogenesis by RT-PCR and showed that this mRNA exists from the earliest stage of oogenesis and at least one third of it is inherited as a maternal mRNA. We then carried out an experiment to deplete the maternal XmN-cadherin mRNA by injecting its antisense S-oligo DNA into full grown oocytes. When mRNA-depleted oocytes were matured in vitro and fertilized eggs obtained therefrom by host transfer technique were allowed to develop, embryos cleaved normally and developed until blastula stage. Such XmN-cadherin mRNA-depleted blastulae initiated invagination, but further involution did not take place, and exogastrulae were formed. These results suggest that the main function of maternally provided XmN-cadherin mRNA is to support cell movement or rearrangement required later during gastrulation, rather than to maintain adhesion of blastomeres during cleavage and blastula formation.

References

Jan 1, 1976·Developmental Biology·R P Lifton, L H Kedes
Dec 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R WinklbauerB Angres
Aug 1, 1991·Mechanisms of Development·F HerzbergD Wedlich
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P DashP Goelet
Oct 1, 1993·The Journal of Cell Biology·P D McCreaB M Gumbiner

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Citations

Jun 27, 2018·Wiley Interdisciplinary Reviews. Developmental Biology·Yunyun Huang, Rudolf Winklbauer
Jun 18, 2010·Developmental Neurobiology·Elisabeth Rungger-BrändleDuri Rungger

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