Exogenous alkaline phosphatase treatment complements endogenous enzyme protection in colonic inflammation and reduces bacterial translocation in rats

Pharmacological Research : the Official Journal of the Italian Pharmacological Society
P Martínez-MoyaFermín Sánchez de Medina

Abstract

Alkaline phosphatase (AP) inactivates bacterial lipopolysaccharide and may therefore be protective. The small intestine and colon express intestinal (IAP) and tissue nonspecific enzyme (TNAP), respectively. The aim of this study was to assess the therapeutic potential of exogenous AP and its complementarity with endogenous enzyme protection in the intestine, as evidenced recently. IAP was given to rats by the oral or intrarectal route (700U/kgday). Oral budesonide (1mg/kgday) was used as a reference treatment. Treatment with intrarectal AP resulted in a 54.5% and 38.0% lower colonic weight and damage score, respectively, and an almost complete normalization of the expression of S100A8, LCN2 and IL-1β (p<0.05). Oral AP was less efficacious, while budesonide had a more pronounced effect on most parameters. Both oral and intrarectal AP counteracted bacterial translocation effectively (78 and 100%, respectively, p<0.05 for the latter), while budesonide failed to exert a positive effect. AP activity was increased in the feces of TNBS colitic animals, associated with augmented sensitivity to the inhibitor levamisole, suggesting enhanced luminal release of this enzyme. This was also observed in the mouse lymphocyte transfer model of c...Continue Reading

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Citations

Feb 11, 2014·Nutrition Reviews·Jean-Paul Lallès
Apr 14, 2016·The Journal of Infectious Diseases·Daniella DeCoffeDeanna L Gibson
Oct 5, 2014·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Cristina MascaraqueFermín Sánchez de Medina
Jul 15, 2015·European Journal of Nutrition·Fermín Capitán-CañadasOlga Martínez-Augustin
Apr 5, 2015·The Journal of Surgical Research·Ben E BiesterveldDavid M Gourlay
Jul 20, 2016·Biochemical Pharmacology·Borja OcónFermín Sánchez de Medina
Nov 12, 2016·Journal of Animal Science and Biotechnology·Jean-Paul Lallès
Oct 21, 2016·Inflammatory Bowel Diseases·Rocío López-PosadasFermín Sánchez de Medina
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Nov 18, 2014·World Journal of Gastroenterology : WJG·Mehrbod EstakiDeanna L Gibson
Jan 28, 2020·American Journal of Veterinary Research·Mahmut OkFunda Terzi
Oct 29, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carlos J ArandaFermín Sánchez de Medina
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Dec 12, 2018·Cancer Chemotherapy and Pharmacology·Kate R SecombeJoanne M Bowen
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Mar 9, 2021·British Journal of Pharmacology·María Arredondo-AmadorFermín Sánchez de Medina

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