Exogenous ascorbic acid enhances vitrification survival of porcine in vitro-developed blastocysts but fails to improve the in vitro embryo production outcomes

Theriogenology
A NohalezC Cuello

Abstract

In this study, the effects of addition of the antioxidant ascorbic acid (AsA) were evaluated during porcine in vitro embryo production (IVP) and vitrification. In experiment 1, the effects of AsA supplementation during IVM, IVF and IVC were evaluated, using a total of 2744 oocytes in six replicates. The IVM, IVF and embryo IVC media were supplemented or not (control) with 50 μg/mL AsA in all possible combinations. No significant effects of AsA were detected in any of the maturation, fertilization or embryo development parameters assessed. In experiment 2, we evaluated the effects of adding AsA to vitrification-warming media on the post-warming survival and quality of blastocysts. Day-6 in vitro-produced blastocysts (N = 588) from six replicates were randomly divided in two groups, with vitrification and warming media either supplemented with 50 μg/mL AsA (VW + group) or un-supplemented (VW- control). Addition of AsA increased (P < 0.05) blastocyst survival rate after vitrification compared with that of VW- control embryos. Vitrification and warming increased (P < 0.05) the production of oxygen species (ROS) and reduced (P < 0.05) the glutathione levels in blastocysts. Although VW + blastocysts displayed higher (P < 0.05) ROS le...Continue Reading

Citations

Jul 16, 2020·Antioxidants·Maria Cristina Budani, Gian Mario Tiboni
Jan 15, 2021·Zygote : the Biology of Gametes and Early Embryos·Sam Zarbakhsh
Jan 9, 2021·Research in Veterinary Science·Yentel Mateo-OteroElisa Giaretta
Dec 3, 2020·Antioxidants·Cristina Rodríguez-Varela, Elena Labarta
Feb 25, 2021·Biopreservation and Biobanking·Du XingzhuFu Xiangwei
Oct 17, 2020·Cryobiology·Baoyu JiaGuoquan Wu
Aug 9, 2021·Theriogenology·Lucy May ViningKatie Evelyn Harvey
Dec 7, 2021·Frontiers in Veterinary Science·Cristina CuelloEmilio A Martinez

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